摘要
采用非离子型去污剂(NP-40)裂解法提取马鹿精子膜蛋白,确定出马鹿精子膜蛋白的最佳分离胶质量分数为12%。通过对膜蛋白的性质分析,发现马鹿精子膜蛋白是一组具弱酸性等电点的低相对分子质量糖蛋白,相对分子质量为21.7～98.4kDa,等电点为pI4～9。
An experiment was conducted to extract sperm membrane proteins from Cervus elaphus by lysis method with non-ionic decontaminant （NP-40）. The optimal concentration of separation gel was determined as 12%. Sperm membrane proteins in C. elaphus were a group of weak acid glyeoproteins with low molecular weight according to the physieoehemical properties of the sperm membrane proteins, and their molecular weights ranged from 21.7 kDa to 98.4 kDa and isoeleetric points were between 4 and 9.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2008年第11期83-85,共3页
Journal of Northeast Forestry University
基金
黑龙江省科技攻关资助项目(GC02B514)
哈尔滨市自然科学基金资助项目(2003AFXXJ016)
东北林业大学优秀青年教师创新资助项目
关键词
马鹿
精子膜蛋白
理化性质
Cervus elaphus
Sperm membrane proteins
Physicochemical properties
