摘要
针对近年来国内外大豆异黄酮的保健功能及结构的相关研究,分析了层析法、高效液相色谱法、高速逆流色谱法分离大豆异黄酮的方法,讨论了紫外分光光度法、高效液相色谱法、高效液相色谱-质谱法、气相色谱法、毛细管电泳法、薄层扫描色谱法、双向纸层析色谱法及免疫法等检测大豆异黄酮的方法及其这些分离检测方法的特点。
The structure and hygienical functions of Soybean isoflavones were summarized in this manuscript, according to the research progress in recent years. And on the basis of reviewing the separation methods of isoflavones from soybean by chromatography and high perfomance liquid chromatography, the detection methods of isoflavones by ultraviolet-visible spectrophotometry, high perfomance liquid chromatography, high perfomance liquid chromatography-Mass spectrometer, gas chromatography, capillary electrophoresis, thin-layer scan chromatography, two- dimensional paper-chromatography and medical immunology were discussed. Furthermore, the characteristic of these separation and detection methods and the main points of operation were reviewed.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2008年第6期6-13,共8页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(30460081)
新疆自治区高校科研计划资助项目(XJEDU2005S15)
石河子大学青年骨干教师资助项目(NX01012)
关键词
大豆异黄酮
分离方法
检测方法
保健功效
soybean isoflavones
method of separation
method of detection
hygienical functions
