摘要
【目的】研究油桃芽体自然休眠诱导期两条主要电子传递途径的运行规律,以期了解主要电子传递途径在落叶果树芽体自然休眠诱导中的可能生理作用。【方法】以油桃品种‘曙光’(Prunus persica var.nectariana cv.Shuguang)为试材,采用呼吸抑制剂法测定休眠诱导期芽体总呼吸速率(Vt)、交替途径发生与实际运行、细胞色素途径与交替途径之间电子配比的动态变化。【结果】花芽和叶芽总呼吸速率变化趋势不一致,花芽呈双峰曲线,叶芽呈单峰曲线。进入休眠诱导期后,芽体交替途径容量(Valt)和交替途径实际运行活性(ρValt)快速上升,但整个测定过程中芽体Valt和ρValt的变化不同步;芽体交替途径实际运行活性对总呼吸的贡献(ρValt/Vt)和细胞色素途径活性对总呼吸的贡献(ρ′Vcyt/Vt)的变化趋势相反,在整个测定过程中,ρ′Vcyt/Vt始终远远高于ρValt/Vt。【结论】交替途径的发生及运行在芽体自然休眠诱导过程中起着极为重要的作用,但细胞色素途径仍是自然休眠诱导期芽体线粒体电子传递的主要途径。
【Objective】Operation regulations of two main electron transport pathways in nectarine buds during endodormancy induction were studied in order to understand the possible roles the two main electron transport pathways played in buds of deciduous fruit trees during endodormancy induction.【Method】Respiration inhibitors（KCN and SHAM） were used to investigate total respiration rate（Vt）,the development and operation of the alternative pathway and the electron partitioning between the cytochrome and alternative pathways in nectarine buds（Prunus persica var.nectariana cv.Shuguang） during endodormancy induction.【Result】Results showed that the variation of Vt in flower and leaf buds showed single and double hump-shaped curves,respectively.In endodormancy induction,the capacity（Valt） and activity（ρValt） of the alternative pathway rapidly increased,but changes of them had different patterns during the measurement.At the same time,the changes of contributions of activities of the alternative（ρValt/Vt） and cytochrome pathway（ρ′Vcyt/Vt） to Vt were opposite,and ρ′Vcyt /Vt was always further higher than ρValt/Vt during the measurement.【Conclusion】All these results suggested that the development and operation of the alternative pathway have played important roles during endodormancy induction,but the cytochrome pathway is the main pathway for mitochondrial electron transport in buds during endodormancy induction.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第12期4149-4154,共6页
Scientia Agricultura Sinica
基金
国家"863"计划项目(2001AA247041)
关键词
油桃芽体
自然休眠诱导
细胞色素途径
交替途径
Nectarine buds,Endodormancy induction,Cytochrome pathway,Alternative pathway
