摘要
为探讨高渗盐／甘露醇液（ＨＳＭ）对内毒素诱导小鼠胸腺细胞凋亡的影响，取昆明纯种小白鼠２４只平分为三组，即①对照组，腹腔注射生理盐水；②内毒素实验组（ＬＰＳ组），腹腔注入内毒素；③ＬＰＳ＋ＨＳＭ组，腹腔注入内毒素前后共注ＨＳＭ二次。结果示，第三组胸腺细胞凋亡百分率及ＤＮＡ断裂百分率明显低于第二组（Ｐ＜０．０１）；ＤＮＡ断裂碎片中的琼脂糖凝胶电泳未见典型的梯状带。提示ＨＳＭ对内毒素诱导的小鼠胸腺细胞凋亡有保护作用；
To observe the effect of HSM (hypertonic saline/mannitol) on thymic cell apoptosis induced by endotoxin (LPS) in mice, twenty-four mice were divided into three gruops (8 mice/each group):1. Control group, each mouse was intraperitonealy injected with normal saline;2. LPS group, each mouse was intraperitonealy injected with LPS;3. LPS+HSM group, each mouse was intraperitonealy injected with HSM before and after injection of LPS.The results showed that the percentage of apoptosis of HSM+LPS group was lower than that of LPS group (P<0.01); the ladder of nucleosomal DNA fragments by agarose gel eletrophoresis of HSM group was not typical as compared with that of LPS group. It is suggested that HSM might protect the apoptosis of mouse thymic cell induced by LPS and may improve the immune function.
出处
《湖南医科大学学报》
CSCD
1998年第1期14-16,共3页
Bulletin of Hunan Medical University
基金
湖南省卫生厅资助
关键词
高渗盐
甘露醇
内毒素
胸腺细胞
细胞凋亡
saline solution,hypertonic
mannitol
endotoxin
cell death
mice
