摘要
利用苯乙酮作为模式底物,对145株菌株进行初筛和复筛,获得一株具有高效立体选择性的酵母菌株YS6-2,能够不对称还原苯乙酮生成(S)-1-苯基乙醇。在苯乙酮浓度为70mmol/L时,底物的初始转化率达26.8%,产物(S)-1-苯基乙醇的对映体过量值为98.8%。基于形态学、生理生化特征、18SrDNA和26SrDNAD1/D2区域的分析表明,YS6-2为胶红酵母(Rhodotorula muci-laginosa)。
Yeast strain YS6-2 with high stereoselectivity was screened from 145 strains when the asymmetric reduction of acetophenone to （S）- 1-phenethylalcohol was chosen as the model reaction. With the concentration of acetophenone at 70 mmol/L, YS6-2 yielded （S）-alcohol at 26.8% conversion and enantiomeric excess reached up to 98.8%. Based on the analysis of 18S rDNA and 26S rDNA D1/D2 domain sequence, along with its general morphology, physiological and biochemical characteristics, YS6-2 was identified as Rhodotorula mucilaginosa.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第12期1920-1925,共6页
Microbiology China
基金
四川省国际科技合作与交流研究计划项目"微生物资源的开发和利用"资助(No.92006H12-020)
