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人骨形态发生蛋白12对人骨肉瘤细胞的生物学作用 被引量：2

Effects of Human Bone Morphogenetic Protein-12 on Osteosarcoma Cell Line MG63 and U2OS
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摘要 为研究人骨形态发生蛋白(human bone morphogenetic proteins,hBMP)12对人骨肉瘤细胞株MG63和U2OS的作用,分别用hBMP12重组腺病毒(AdBMP12)以及含重组hBMP12(recombinant hBMP12,rhBMP12)的条件培养液干预人骨肉瘤细胞MG63和U2OS,利用台盼蓝拒染法、TUNEL法、吖啶橙/溴乙啶(AO/EB)荧光双染法、Transwell小室和碱性磷酸酶活性测定法分别检测细胞增殖、凋亡、迁移以及成骨分化能力的变化.与相应对照组相比,AdBMP12和含rhBMP12的条件培养液的干预致两种骨肉瘤细胞株细胞存活率降低,并呈一定的时间依赖性;凋亡率均随时间延长而增加,并且两种检测方法的结果一致;不同时间点的细胞穿膜数均明显减低;碱性磷酸酶活性在干预3d后开始逐渐增加,至第9d仍可观测到.以上差异均有统计学意义(P<0.01).提示无论是以腺病毒介导基因转入还是重组蛋白直接作用方式,hBMP12都可以抑制人骨肉瘤细胞株MG63和U2OS的增殖和迁移,并诱导其凋亡和向成骨细胞分化. In order to investigate the effects of hBMP12 on osteosarcoma cell MG63 and U2OS, Adenovirus-BMP12 （Ad-BMP12） and recombinant human BMP12-conditioned media （rhBMP12-CM） were used to treat the cell lines. Their proliferation, apoptosis, and transmigration were detected by Trypan blue exclusion test, TdT-mediated biotinylated-dUTP nick end labling （TUNEL）, acridine orange-ethidium bromide （AO/EB） double fluorescent dye staining, and transwell-room test, respectively. The alkaline phosphatase （ALP） activity was detected to reflect the differentiation of tumors. Compared with the control groups, the cell survival rates of experimental groups which treated with AdBMP12 or rhBMP12-CM showed a significant time-dependent decrease （P〈 0.01 ）. The apoptosis indexes were increased significantly （P〈 0.01 ） and the results from TUNEL and AO/EB method were consistent. The cell numbers of transmembrane significantly decreased at three detecting time points for the two cell lines （P〈 0.01 ）. BMP12 treatment enhanced the activity of ALP of both cell lines within 3 days and this effect was observed at least up to day 9 of the treatment （P〈0.01）. In conclusion, hBMP12 could inhibit the proliferation and transmigration of osteosarcoma cells, induce their apoptosis and differentiate into osteoblasts in osteosarcoma cell lines MG63 and U2OS.
作者 李星星 何焕玲 吴丽美 左国伟 苗静琨 周兰
机构地区 重庆医科大学生物医学工程系重庆市生物医学工程学重点实验室重庆市超声医学工程重点实验室--省部共建国家重点实验室培育基地
出处 《生命科学研究》 CAS CSCD 2008年第4期365-372,共8页 Life Science Research
基金 重庆市自然科学基金资助项目(CSCT2005BB5320)
关键词 骨形态发生蛋白 细胞增殖 细胞凋亡 细胞迁移 碱性磷酸酶活性 bone morphogenetic protein proliferation transmigration apoptosis the activity of alkaline phosphatase
分类号 R738.1 [医药卫生—肿瘤]
  • 相关文献

参考文献11

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共引文献26

同被引文献22

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生命科学研究
2008年 第4期

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