摘要
目的:研究人工合成抗菌肽对大肠杆菌生物膜的影响。方法:采用试管法建立大肠杆菌生物膜,将大肠杆菌生物膜随机分成4个组A组:样本中加入4×MIC抗菌肽;B组样本中加入1×MIC庆大霉素;C组样本中联合加入4×MIC抗菌肽和1×MIC庆大霉素;D组样本中加入PBS液作为空白对照。处理6h后对样本进行活菌计数,并利用扫描电镜观察比较处理组与对照组间差异。结果:6h后活菌计数,C组与其他三组相比活菌数明显减少(P≤0.05);而A组和B组与D组间比较活菌数减少(P≤0.05)。扫描电镜观察可见A组与C组中生物膜内大肠杆菌的细胞膜完整性受到破坏。结论抗菌肽与庆大霉素联合运用时的杀菌效果显著增强。
Objective： To study the effect of synthetic antimicrobial peptides on Escherichia coli biofilm. Methods： The test tube method was used to establish the model of Escherichia coli biofilm. The samples were randomly divided into four groups： group A,by adding 4 × MIC antimicrobial peptides to the samples,group B,by adding 1 x MIC gentamicin to the samples,group C,by adding 4 × MIC antimicrobial peptides and 1 × MIC gentamicin to the samples,and group D,by adding phosphate buffered saline to the samples as a control. After 6 h the viable bacteria in the biofilm were counted following the method of colony count. The different changes in the biofilm were compared between the treated groups and the control group with scanning electron microscope. Results： The viable bacteria in group C were significantly decreased compared with the other three groups（P〈0.05）. While compared with group D,the viable bacteria were decreased in group A and group B（P~〈0.05）. From the graph of scanning electron microscope the integrity of the Escherichia coli cell membrane was disrupted in group A and C. Conclusion： The results suggest that the killing effect of combination of antimicrobial peptides with gentamicin is significantly increased.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第12期1492-1494,共3页
Journal of Chongqing Medical University
关键词
抗菌肽
庆大霉素
生物膜
大肠杆菌
Antimicrobial peptides
Gentamicin
Biofilm
Escherichia coli
