摘要
在建立宁夏枸杞果实总黄酮的快速检测方法的基础上分析我国各大宁夏枸杞产区的枸杞子总黄酮含量差异,并初步鉴别主要黄酮。以芦丁作为对照品,以石油醚脱色,70%乙醇微波提取,在258nm处直接测定。该测定方法在5.0μg/mL～15.0μg/mL浓度范围可以准确、快速地测定提取液中的总黄酮,准确度和回收率分别在99.13%和99.77%以上。中宁县作为宁夏枸杞的道地产区,枸杞子的总黄酮含量最高,各主要产区的果实总黄酮含量高低次序为:中宁>沙海>诺木洪>奇台>精河。采用色谱柱分离总黄酮和化学法鉴定主要黄酮,提取液的主要黄酮是一种异黄酮。
A direct ultraviolet spectrophotometric method for quantitation of total flavonoids from the fruits of Lycium barbarum L. was established： the deoiled samples were extracted with 70 % ehtanol, and then measured at 258 nm, with rutin as eqivalents; accuracy and recovery were respectively over 99.13 % and 99.77 %. Through separation by HPLC and coloration by chemical reagents, the main flavonoids from fruits ofL. barbarum L. were preliminarily identified as isoflavones. Analysis on the contents of total flavonoids of fruits from 6 producing areas indicated that Zhongning （Ningxia,China） is the genuine（＂Daodi＂） producing area for L. barbarum L.
出处
《食品研究与开发》
CAS
北大核心
2009年第1期36-40,共5页
Food Research and Development
基金
中国科学院百人计划基金(05045112)
武汉市农业局茎叶类蔬菜的开发利用项目(200720322099)
中国科学院知识创新工程重要方向项目(KSCX2-YW-N-030)
关键词
宁夏枸杞
总黄酮
含量
分离
鉴别
异黄酮
Lycium barbarum L.
total flavonoids
quantitation
separation
identification
isoflavones
