摘要
为建立鸡蛋中三聚氰胺残留的简便高效液相色谱法，本试验采用NH2柱作为色谱分析柱，90％乙腈水溶液为流动相，鸡蛋样品经混合均匀后，用三氯乙酸-乙腈溶液提取，经阳离子交换固相萃取柱净化、富集后，在紫外检测器230nm处用高效液相色谱仪测定，保留时间在6～7min。标准曲线在0．1～5．0μg／ml浓度内呈线性相关，相关系数（r）为0．9999。在空白鸡蛋样品中添加0．2mg／kg、0．5mg／kg、2．0mg/kg和5．0mg／kg的三聚氰胺，回收率为78．96％～92．19％，相对标准偏差（RSD）为1．96％～6．60％。样品检测定量限为0．2mng／kg。
A high-performance liquid chromatography （HPLC） with NH2 column and 90% acetonitrile eluate to determine the melamine in eggs was developed. Samples were extracted with 1% trichloroacetic acid-acetonitrile. Target analyres of samples were cleaned up and concentrated by HyperSep Retain-CX column solid-phase extraction （SPE）. A perfect separation for melamine was achieved and retention time was about 6 - 7 min at 230 nm. The calibration curve was linear in a concentration range of 0. 1 - 5.0 g/ml, with correlation coefficient of 0. 999 9. Recoveries of melamine at fortified levels of 0. 2 mg/kg, 0. 5 mg/kg, 2. 0 mg/kg and 5.0 mg/kg ranged from 78.96% -92. 19%, with relative standard deviation of 1.96% - 6. 60%. The quantification limit was 0. 2 mg/kg.
出处
《江苏农业学报》
CSCD
北大核心
2008年第6期936-939,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家“十一五”支撑计划(2006BAK02A28)
江苏省创新专项[CX(08)601]
关键词
鸡蛋
三聚氰胺
残留
高效液相色谱法
egg
melamine
residue
high-performance liquid chromatography
