摘要
生物测定了球孢白僵菌（Beauveria bassiana）Bb050715，Bb060721菌株和粉拟青霉（Paecilomyces farinasus）Pf0607菌株对松墨天牛（Monochamus ahernatus Hope）的感染效应。结果表明，球孢白僵菌及粉拟青霉对松墨天牛幼虫的毒力有一定的差异，3株丝孢真菌侵染三龄幼虫后，均造成虫体血淋巴中蛋白质含量降低，且球孢白僵菌侵染后血淋巴中总蛋白含量明显低于经粉拟青霉接种处理感染后的虫体。供试3菌株中，球孢白僵菌菌株处理后天牛累积死亡率明显高于粉拟青霉菌株，对天牛幼虫感染的剂量效应参数分别为0．45，0．45和0．39，对松墨天牛三龄幼虫的毒力回归方程分别为Y=13．537X-4．6032（R^2=0．97），Y=15．361X＋0．4444（R^2=0．97）和Y=9．138X-3．1001（R^2=0．99）。在1．36×10^4～1．36×10^9孢子／mL下处理后第4d才出现死亡，Bb050715，Bb060721和粉拟青霉3菌株接种处理后第4～8d的累积死亡率分别为13．3％～80．0％，13．3％～86．7％，6．7％-53．3％，LT50依次为5．1，5．1和7．7d，第5d的LC50分别为7．23×10^8孢子／mL，1．53×10^8孢子／mL，7．27×10^9孢子／mL。
The infection effect of isolates of Bb050715 and Bb060721 strains of Beauveria bassiana, and the strain of Pf0607 of Paecilomyces farinasus against the larvae of pine sawyers, Monochamus alternatus Hope were tested in laboratory. The result showed that the virulence was different between B. bassiana and P. farinasus, and the protein content in haemolymph of the 3rd instar larvae of the M. alternatus infected by the isolates of B. bassiana decreased obviously and was less than that of the larvae infect by P. farinasus. The cumulative mortality of the 3rd instar larvae of the M. alternatus infected by the isolates of B. bassiana were obviously higher than that of the larvae infect by P. farinasus. The dose - effect parameters of the tested isolates were 0. 45, 0. 45 and 0.39, respectively, on larvae of the M. alternatus. The virulence equation was Y = 13.537X - 4. 603 2 （ R^2 = 0. 97 ） , Y = 15. 361X ＋0. 4444 （R^2 =0. 97） and Y=9. 138X -3. 100 1 （R^2 =0. 99）, respectively. The LT50 was 5.1, 5.1 and 7.7 days, separately. Its LC50 on the larvae against sawyer was 1.38 × 10 spores/mL.The cumulative mortalities were 13.3% -80%, 13.3% -86.7%, 6.7% -53.3%, respectively, after the inoculation of the dosage of 1.36× 10 ^4 1.36 × 10^9 spores/mL, and the first mortality was in the 4th day after treatment. The LC50 in the day 5 was 7.23 × 10^8 spores/mL, 1.53 × 10^8 spores/mL, 7.27 × 10^9 spores/mL.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第1期47-50,66,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
国家自然科学基金项目(30860005)
云南省科技厅重点攻关项目(2005NG03)
云南省自然科学基金项目(2008CO65M)
关键词
松墨天牛
球孢白僵菌
粉拟青霉
蛋白质含量
毒力
Monochamus alternatus
Beauveria bassiana
Paecilomyces farinasus
protein content
virulence
