摘要
以野生春兰茎尖培养所得根状茎为材料,用102、03、04、0 Gy60Coγ-射线进行辐照,以不经辐照为对照。处理材料培养在1/2MS+NAA 5 mg/L上进行增殖,经30 d后统计重量及生长点数,同时转入1/2MS+NAA 0.2 mg/L+BA 3 mg/L进行分化培养,经45 d后统计分化出的苗数。结果表明:随着辐照剂量增大,根状茎的增殖特别是新生长点的形成受抑制,苗分化均略有促进,但未达显著水平,高剂量(>30 Gy)将严重抑制增殖与分化,20 Gy应为春兰根状茎辐照诱变的最适剂量。EMS处理分别以0.6%、0.8%浸泡6 h和8 h,以磷酸缓冲液浸泡为对照,结果表明,处理与对照差异显著,随着浓度的增大,根状茎的死亡率明显提高,0.8%浸泡6 h与8 h之间差异不显著。
^60Coγ-Ray radiated on rhizomes of Cymbidium goeringii at four dose, there were 10, 20, 30, 40 Gy. The rhizomes were proliferated on 1/2MS＋NAA5 mg/L for a month. Then they were transferred to differentiation media, 1/2 MS＋NAA 0.2 mg/L＋BA 3 mg/L. After 45 days, the weight of rhizomes and buds＇ number were investigated. The results showed that low dose of ^60Coγ-Ray （〈10 Gy）can promote the multiplication and differentiation, but its affect was not salience, high dose （〉30 Gy） would restrain the multiplication and differentiation severity on the other hand. The optimum treatment dose for breading was 20 Gy. On the other hand, rhizomes were dipped in 0.6%, 0.8% ethyl methanesulfonate （EMS） solution for 6 or 8 hours, then there were transferred to medium. The results were compared with it of phosphate buffer solution. It revealed that EMS could restrain its growth speed, and the mortality was raising with the increasing of EMS concentration.
出处
《北方园艺》
CAS
北大核心
2009年第1期49-52,共4页
Northern Horticulture
基金
上海市科技兴农重点攻关资助项目[沪农科攻字(2003)第4-3号]
关键词
春兰
根状茎
60Co
EMS
Cymbidium goeringii
Rhizomes
^60Coγ-Ray
Ethyl methanesulfonate
