摘要
EST是指从EST cDNA文库中随机挑取克隆进行单轮自动测序所获得的短的、常常是未加编译的cDNA序列。目前,大约有200种植物共超过1500多万条的EST的序列,研究表明对EST进行大规模研究已成为功能基因组学研究的最佳途经之一。分析和比较EST序列就可以得到关于基因的表达、功能及进化信息,利用大量的EST序列已经成为发现新基因及了解植物新陈代谢一种简便有效的方法。通过介绍基于植物表达序列标签(EST)的微卫星(SSR)标记的研究现状,并对一些植物中利用EST-SSR标记在遗传图谱构建、遗传多样性、通用性等方面的应用进行了综合评述。同时也提出利用EST存在的一些不足及发展趋势。
Expressed sequence tags （ESTs） are short, usually unedited sequences obtained by single-pass sequencing of cDNA clones from any cDNA library. Currently, plant EST collections comprise over 15 million sequences from about 200 species. A number of studies indicate that EST analysis is one of the most effective approaches to explore big genomes. Analyzing and comparing ESTs can provide in formation on gene expression, function and evolution. Large-scale EST sequence has become an attractive alternative to discovery plant genome sequence and plant metabolism analysis. The research status based on the plant EST-SSR markers was introduced, and the genetic map construction, genetic diversity and generality by using the EST-SSR technology in some crops were also reviewed. At the same time, EST also made use of a number of deficiencies and development trends.
出处
《分子植物育种》
CAS
CSCD
2009年第1期125-129,共5页
Molecular Plant Breeding
基金
国家自然科学基金(30660113)资助
