摘要
本文以银杏幼苗茎段为外植体诱导产生愈伤组织，测定了其中的黄酮类化合物的积累量，并初步探索了愈伤组织生长与黄酮类化合物积累的关系和影响积累的有关因素。研究结果表明：诱导幼苗茎段愈伤组织产生的最适培养基为ＭＳ＋２．５ｍｇ／ＬＮＡＡ＋１ｍｇ／ＬＫＴ＋２００ｍｇ／ＬＬＨ＋３０ｇ／Ｌ蔗糖；愈伤组织生长周期约３０ｄ；为使愈伤组织维持较长时间的生长，要在其生长２５～３０ｄ时用中等盐分浓度的基本培养基继代培养；光下诱导的愈伤组织中有黄酮类化合物产生；总量最高为１．０ｍｇ／ｇＤＷ；其中槲皮素甙元为１７．５μｇ／ｇＤＷ，０．５ｍｇ／Ｌ２，４－Ｄ＋１ｍｇ／ＬＫＴ，０．５ｍｇ／ＬＧＡ３＋１ｍｇ／ＬＫＴ有利于黄酮类化合物的积累；愈伤组织生长与黄酮类化合物积累两者趋势大体一致；ＮＨ＋４的浓度与黄酮类化合物产生有关。
Young stem of Ginkgo biloba were used to induce callus,and the relation between callus growth and flavonoids accumulation were studied.The result indicated that the ideal medium for a large volume of callus induction is MS supplemented with 2.5 mg/L 1 Naphthaleneacetic acid and 1 mg/L kinetin.In order to subculture callus for several cycles,it is suitable to subculture the callus by using middle salt concentration medium in 25～30 days.The flavonoids were identified in the callus cultured under light.The supreme quantity of flavonoids reached to 1.2 mg/gDW,within it there were 17.5 μg/gDW quercetin.Production of flavonoids was related to callus growth,and the con.of NH + 4 was an effective factor.
出处
《林业科学研究》
CSCD
北大核心
1998年第2期124-129,共6页
Forest Research
基金
林业部亚热带林木培育重点开放实验室基金
