摘要
采用PHA、秋水仙素碱腹腔或背部肌肉注射，活体培养法，用空气干燥法制片，运用Micromeasure version3．3染色体分析软件和Photoshop7．0对似刺鳊鮈染色体数目和核型进行了分析；同时，取似刺鳊鮈血细胞、尾鳍、鳃、肌肉、性腺和肝脏为材料，以鸡血细胞为DNA标准（2．50pg／2c），使用EPICS-XL型流式细胞仪测定了似刺鳊鮈细胞核DNA含量。结果显示：染色体众数2n=50，染色体大小的绝对值为0．86～2．32μm，平均长度为1．63μm，未观察到次缢痕及异配型染色体，亦未发现有随体，核型公式为18m＋20sm＋8st＋4t，NF=88。6个组织细胞核DNA含量分别为：尾鳍3．779pg／2c，鳃4．007pg／2c，肌肉3．819pg／2c，性腺（卵巢4．242pg／2c、精巢1．842pg／2c），肝脏3．905pg／2c。尾鳍、鳃、肌肉和肝脏的细胞核DNA含量不存在显著差异（P〉0．05），但都极显著高于血细胞DNA含量（P〈0．01）；卵巢细胞核DNA含量约为精巢的2倍。
The metaphase chromosome number and karyotype of Paracanthobrama guichenoti were analyzed using chromosome analysis software （ Micromeasure version 3.3） and Photoshop 7.0. Kidney cells treated with PHA and colchicine through intramuscular and intra-peritoneal injections were cultivated, and finally were sliced by air-drying method. The cellular DNA contents of Paracanthobrama guichenoti were determined using Flow cytometer （EPICS-XL） based on blood corpuscle and the ceils from tail fin, gill, muscle, gonad and liver of fish compared with standard DNA of chicken erythrocyte （2.50 pg/2c）. The results indicate that the telocentric chromosome number of Paracanthobrama guichenoti is 50 （2n = 50） and the absolute value of chromosome size ranges from 0.86 -2.32 μm with the average value of 1.63 μm. The karyotype of Paracanthobrama guichenoti is 2n = 18m ＋ 20sm ＋ 8st ＋ 4t, NF = 88. However, secondary constriction, heterozygosity chromesome and satellite chromesome were not observed. The cellular DNA contents of tissue from tail fin, gill, muscle, ovary, spermary and liver are 3. 779 pg/2c,4. 007 pg/2c, 3.819 pg/2c,4. 242 pg/2c, 1. 842 pg/2c, and 3. 905 pg/2c respectively. Moreover, the cellular DNA contents are not significantly different in tail fin, gill, muscle and liver of Paracanthobrama guichenoti （P 〉 0.05）, but they are significantly higher than the cellular DNA contents in erythrocyte （ P 〈 0.01 ）. In addition to above, the cellular DNA contents in ovary are twice as great as those in spermary.
出处
《水产学报》
CAS
CSCD
北大核心
2009年第1期9-14,共6页
Journal of Fisheries of China
基金
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院淡水渔业研究中心)资助项目(2007JBFB03)
农业部水生动物遗传育种和养殖生物学重点开放实验室开放课题项目(BM2007-08)
中国水产科学研究院淡水生态与健康养殖重点开放实验室开放课题项目(2007FEA0203)共同资助
关键词
似刺鳊鮈
染色体
核型
DNA含量
Paracanthobrama guichenoti Bleeker
chromosomes
karyotype
DNA content
