摘要
研究两种红树植物海莲和木榄幼苗叶片在不同培养盐分处理下，蛋白质含量、超氧化物歧化酶及膜脂质过氧化作用的变化情况．结果表明：随着盐分的增加，幼叶的蛋白质含量在总体上呈下降的趋势，海莲从对照的２．２ｍｇ／ｇＦＷ下降到３０‰盐度时的０．８ｍｇ／ＦＷ，木榄的蛋白质变化则较为复杂．具有膜保护作用的超氧化物歧化酶活性在总体上则逐步随盐度增加而增加，海莲幼叶的ＳＯＤ活性从对照的３１个活性单位／ｍｇＰｒ上升到３０‰盐度的９５个活性单位／ｍｇＰｒ，木榄的ＳＯＤ活性升高则更为明显．与ＳＯＤ变化相反，膜脂质过氧化作用随盐度升高则表现出较为明显的下降趋势（木榄）和在低盐度下降，高盐度上升的变化（海莲）．讨论了海莲和木榄幼叶在不同盐度条件下膜脂质过氧化作用和膜保护酶系统的关系，表明脂质过氧化作用和ＳＯＤ保护作用是盐胁迫的主要过程，可作为红树植物耐盐性的参数．
The variations of protein content, activity of superoxide dismutase (SOD) and membrane lipid peroxidation intensity in leaves of B.sexangula and B.gymnorrhiza seedlings with the changes of salinity were investigated.The results showed that the contents of protein in leaves for both mangrove seedlings decreased generally with increasing of culture salinity.e.g.the protein content in B. sexangula seedling leaf increased from 2.2 mg/g FW for control to 0.8 mg/ g FW for 30 ‰ salinity treatment.The activity of SOD which is the key enzyme for membrane protection system increased in parallel with increasing of salinity.For example, the SOD activity in B.sexangula seedling leaf increased from 31 unit/mg Pr for control to 95 unit/ mgPr for 30 ‰ salinity treatment.Futhermore, the increasing of SOjD activity in B. gymnorrhzia was more obvious. On the contrary, lipid peroxidation intensity reduced obviously with increasing salinity for B.gymnorrhiza and reduced first in slight salinity then increased in severe salinity for B. sexangula . The realtionship between peroxidation and membrane protection enzyme was discussed for both mangrove plant seedlings at different culture salinities. The process of lipid peroxidation in seedling leaf and SOD protection for membrane system was the key process on salinity stress,and it could be characterized as salt tolerance in mangrove plant seedling.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第2期278-282,共5页
Journal of Xiamen University：Natural Science
关键词
红树林
海莲
木榄
盐度
幼苗
膜保护系统
Salt stress,Mangrove, B.sexangula,B.gymnorrhiza, Lipid peroxidation,MDA,SOD
