摘要
目的探讨红景天对阿尔茨海默病(AD)模型大鼠的干预效果和作用机理。方法采用腹腔注射D-半乳糖(用生理盐水配成浓度为10mg/mL)和灌胃AlCl3(用蒸馏水配成浓度为20mg/mL),并一次性注射东莨菪碱建立AD动物模型。通过Morris水迷宫测试系统检测大鼠学习记忆能力变化,抗超氧阴离子和总抗氧化能力测定检测各组大鼠脑组织抗氧化水平,常规HE染色和免疫组化测Aβ1-40的表达,观察大鼠海马及皮质的形态学改变。用红景天以灌胃方式对实验大鼠进行干预,并以喜得镇做对照观察其干预效果。结果模型组大鼠记忆功能明显减退,神经细胞破坏明显,海马和皮质出现β-淀粉样蛋白沉积及老年斑。中药组、西药组及空白组大鼠在水迷宫测试中,逃避潜伏期明显缩短,原平台象限比、跨平台次数明显增加,与模型组比较差异有统计学意义(P<0.05);模型组抗超氧阴离子和总抗氧化能力活性较其他组明显降低(P<0.05)。结论红景天可以有效改善AD大鼠空间学习记忆能力损害,同时在清除自由基和抗氧化损伤方面有显著功效,具有神经保护作用。
Objective To study the intervention effect and mechanism of integripetal rhodiola herb （IRH） on Alzheimer＇s disease model rats. Methods Alzheimer＇s disease model was made by injecting D-galactose （10 mg/mL） in abdominal cavity and talcing AlCl3 （20 mg/mL） by intragastric administration, and injecting scopolamine. Morris water maze testing system was undertaken to observe the change of learning and memory ability in rats, biochemical indicator tested was to contrast the state of oxidative stress, common HE staining and immunohistchemistry staining were to observe the morphology change of the hippcampus and cortex cell. The model rats were administrated IRH by means of intragastric administration contrasting with hydergine to compare the effect. Results The memory ability of model group was obviously decreased, ceUula nervosa were damaged badly. Aβ and senile plaques appeared on hippcampus and cortex. All of the test indexes in Morris water maze showed that the abilities of space search, learning and memory in different groups remarkably improved compared with that of the model group （P 〈0.05）. The anti-O2 ·- and T-AC activity of the model group was lower than other groups obviously （P〈0.05）. Conclusion IRI-I has significant relieving effect on the lesion of the abilities of space search, learning and memory in AD model rats and good protecting effect on cellula nervosa of AD rats by eliminating free radical and improving the anti-oxydation potential.
出处
《中国中医药信息杂志》
CAS
CSCD
2009年第2期38-40,共3页
Chinese Journal of Information on Traditional Chinese Medicine
关键词
红景天
阿尔茨海默病
行为学
抗氧化能力
大鼠
integripetal rhodiolaherb Alzheimer＇s disease
ethology anti-oxydation potential
rat
