摘要
目的:研究高眼压条件对大鼠视网膜神经感觉层(neurosensory retina)超微结构的影响。方法:利用自制的加压装置使大鼠眼内压升高,维持眼底缺血状态2h,再分别于恢复血液再灌注0,8,24,48h;7d处死大鼠,取出视网膜组织,电镜观察。结果:电镜下观察再灌注8h视网膜外核层、内核层细胞死亡表现出凋亡和坏死两种特征,神经纤维层水肿明显,细胞线粒体出现空泡样结构,24h有视网膜节细胞凋亡数量最多,48h减少,7d后凋亡细胞数量减少,细胞密度减小,视网膜萎缩变薄。结论:急性高眼压条件下视网膜各层细胞易损性不同,缺血再灌注不同时间各层超微结构变化不一致,凋亡不仅是视网膜节细胞的死亡形式也是其它层细胞的死亡形式。
AIM. to investigate the ultrastructural changes of neurosensory retina cells under acute intraocular hypertension condition. METHODS： the experimental ocular hypertension rat was established by self-made pressure transmission apparatus. The ischemic neurosensory retina kept 2 hours and were taken out after the rats were killed in blood reperfusion 0,8,24,48 hours and 7 days. The specimen were fixed, sliced and observed with electron microscope. RESULTS, after 8 hours reperfusing, apoptosis occurred in outer nuclear layer, edema and vacuole-like structure in mitochondria were appeared in nerve fiber layer. Apoptosis of retinal ganglion cells reached to the peak after 24 hours reperfusion and reduced after 48 hours. On 7^th day, we found atrophy of retina, which had low density of cell and few apoptosis cells. CONCLUSION. all of the anatomic layers of neurosensory retina in acute intraocular hypertension may suffer from degeneration and atrophy at different degrees with different ultrastructural changes. Not only retinal ganglion cells but cells in other layers are dead in apoptosis procedure.
出处
《国际眼科杂志》
CAS
2009年第1期49-51,共3页
International Eye Science
基金
中国陕西省科技计划项目(No.2004K10-G7)~~
关键词
急性高眼压
视网膜神经感觉层
大鼠
超微结构
acute intraocular hypertension
neurosensory retina
rats
ultrastructure
