摘要
为了得到右旋的2-氨基丁酸，对2-氨基丁酸的拆分进行了研究．通过从猪肾中提取的氨基酰化酶选择性地水解（R，S）-2-N-乙酰氨基丁酸，得到了（S）-2-氨基丁酸，旋光度=＋21．58℃（C=2，5mol／LHCl），收率为71．8％．试验结果表明最佳拆分条件为：底物浓度为0．1mol／L；底物与酶质量比为100：1；pH6．8～7．0；温度为37℃；反应时间为24h．
A study was made on the resolution of 2-aminobutyric acid in order to obtain dextrogyarte 2- aminobutyric acid. In our experiment, （S）-2-aminobutyric acid was resolved from （R, S）-2-aminobutyric acid by selective hydrolyzation of （R, S）-2-N-acyl aminobutyric acid which in the presence of acylase was derived from the kidney of pigs. The optical rotation= ＋21.58℃= 2,5N HCl） and the yield of （S）-2-aminobutyric acid is 71.8%. The experimental results showed that the best hydrolytic conditions are as follows： the concentration of the substrate is controlled to about 0. 1 mol/L;m（subsrate）：m （enzyme） = 100 ： 1 ; pH value of reaction system is 6.8 - 7.0 ; reaction temperature is 37℃ and reaction time is 24 h.
出处
《武汉工程大学学报》
CAS
2009年第3期26-29,共4页
Journal of Wuhan Institute of Technology
