摘要
目的:建立一种新的快速有效的测定八角挥发性成分的分析方法。方法:采用静态顶空-气相色谱-质谱法测定八角果实和叶片中的挥发性成分,采用面积归一法计算各组分的相对含量。结果:分别从八角果实和叶片的顶空气体中鉴定出了36种和37种化合物,被鉴定挥发性组分的峰面积分别占总峰面积的97.62%和99.68%;在八角果实和叶片的顶空气体中,均是以芳香族化合物的相对含量最高,占总挥发性物质的82.3%～90.2%,其次为萜类化合物,占7.3%～17.3%,非萜类脂肪族化合物的相对含量最低仅占0.4%～2.4%;分析结果的RSD在2.11%～6.80%之间。结论:本方法具有较高的精密度,且样品消耗量小,无需有机溶剂提取,整个过程高度自动化,可快速检测与区分八角果实和叶片所得挥发油,亦可用作八角挥发油提取原料的质量控制。
Objective：To establish a fast and effective new method for the analysis of the volatile compounds from Illicium verum Hook. f.. Methods： The volatile compounds in fruit and leaf from lllicium verum were analyzed by static headspace - GC - MS, then separated and identified by GC - MS. Relative content of each volatile compounds was determined by area normalization. Results：There were 36 and 37 species of volatile compounds were identified in the headspace in fruit and leaf from Illicium verum,97.62% and 99.68% of the peak area were identified from the total volatile compounds peak area in the headspace of fruit and leaf. In the headspaee,82. 3% -90. 2% of the peak area of volatile compounds was contributed by aromatic compounds,7.3% -17.3% of the peak area was contributed by terpenes, and there were only 0.4% - 2.4% of the peak area was contributed by aliphatic compounds. RSD of this method was in the range of 2. 11% -6. 80%. Conclusion：This method has many advantages in analysis of volatile compounds from Illicium verum, with high precision, less sample consumption, no organic solvent extraction, and highly automatic analysis process. It can be applied for fast analysis and distinguish of the volatile compounds in fruit and leaf from Illicium verum, as well as in controlling the quality of materials by extract star anise oil.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第1期91-95,共5页
Chinese Journal of Pharmaceutical Analysis
基金
国家科技攻关计划"西部开发"重大项目(2005BA901A09)
关键词
八角
静态顶空分析
气相色谱-质谱分析
挥发性成分
Illicium verum Hook. f.
static headspace analysis
gas chromatography - mass spectrometry analysis
volatile compound
