摘要
采用SPAD-502叶绿素计、LI-6400型便携式光合分析仪与分光光度法分别对种子源于江西遂川(MPS)和福建建瓯(MPJ)的3年生刨花楠与江西上犹(MLG)的3年生华东润楠苗木叶片的SPAD值、净光合速率(Pn)和叶绿素a+b含量(Ct)进行了测定.结果表明:3种苗木叶片叶绿素含量存在显著差异,其SPAD值、叶绿素a+b、叶绿素a与叶绿素b含量均为MPS<MLG<MPJ,其SPAD均值分别为43.80、45.12和50.67,叶绿素a+b含量分别为1.944、2.831和3.447mg·g-1.受叶肉组织发育成熟程度影响,当年生新叶的叶绿素含量低于2年生叶片.MPS与MPJ刨花楠同一叶龄叶片不同冠层间的Ct次序为上冠层<中冠层<下冠层,华东润楠则为上冠层<下冠层<中冠层.同一叶片不同观测部位间的SPAD值次序为尖端<中部<基部.叶片的SPAD值与叶绿素a+b含量呈显著正相关,与Pn虽存在正相关,但不显著.
By using SPAD-502 chlorophyll meter, LI-6400 portable photosynthesis system, and spectrophotometer, the leaf SPAD value, net photosynthetic rate content （ Ct ） of 3-year-old Machilus pauhoi and M. leptophylla （Pn）, seedlings and chlorophyll （ a ＋ b） were measured, and the relationships of SPAD value with Pn and C, were analyzed. The M. pauhoi seedlings were grown from the seeds originated from Suichuan County of Jiangxi Province and Jian＇ ou County of Fujian Province, named as MPS and MPJ, respectively; while the M. leptophyUa seedlings were grown from the seeds originated from Shangyou County of Jiangxi Province, named as MLG. There were significant differences in the mean chlorophyll content of MPS, MPJ, and MLG. The SPAD value and the contents of chlorophyll （ a ＋ b） （ Ct ）, chlorophyll a （ Ct ） and chlorophyll b （ Cb） were in the order of MPS 〈 MLG 〈 MPJ, with the mean SPAD value being 43.80, 45.12, and 50. 67 and the Ct value being 1. 944, 2. 831, and 3.447 mg · g^-1, respectively. The chlorophyll content was influenced by the maturing degree of mesophyll tissues of M. pauhoi and M. leptophylla, being lower in current-year leaves than in 2-year-old leaves. The Ct of same age leaves at different crown layers of MPS and MPJ and of MLG was in the order of upper layer 〈 middle layer 〈 lower layer and of upper layer 〈 lower layer 〈 middle layer, respectively, and the SPAD value of the same lamina at different positions was in the order of apex 〈 middle 〈 base. SPAD value had a significant positive linear correlation with Ct, and a statistically not significant positive correlation with Pn.
出处
《应用生态学报》
CAS
CSCD
北大核心
2009年第2期271-276,共6页
Chinese Journal of Applied Ecology
基金
福建省科技厅重点项目(2004N010,2005NZ1010)
福建省重点学科建设资助项目(12311009)
关键词
SPAD值
叶绿素含量
净光合速率
刨花楠
华东润楠
SPAD value
chlorophyll content
net photosynthetic rate
Machilus pauhoi
Machilus leptophylla.
