摘要
本研究探讨丹参酮Ⅱa对家兔免疫性血管炎的炎性细胞因子IL-1β、IL-6和TNF-α的影响及其作用机制。建立免疫性血管炎模型,实验分为正常组、模型组、丹参酮Ⅱa治疗组和阿司匹林治疗组。采用ELISA法检测治疗后血清IL-1β、IL-6和TNF-α水平。应用HE染色、弹力纤维染色和扫描电镜观察血管病变。结果表明:急性期模型组细胞因子IL-1β和TNF-α水平较正常组明显增高(p<0.05),细胞因子IL-6水平在各组之间尚未见无显著性差异。相关分析显示:血小板数目与IL-1β水平有一定程度的相关,血小板聚集功能则与IL-1β、TNF-α水平有关。丹参酮Ⅱa治疗可以明显降低上述各项指标的水平,并且其作用效果与阿司匹林基本相当。两者均可以显著减轻炎症对血管的病理损害。炎性细胞因子IL-1β和TNF-α参与了血管炎发病机理,并与血小板数目和功能相关。结论:丹参酮Ⅱa可能通过抑制炎性细胞因子以及抑制血小板作用从而达到抗炎治疗、减轻病理损害的作用。
The objective of this study was to investigate the effect of Tanshinone Ha on IL-1β, IL-6 and TNF-α cytokines in immune vasculitis and platelets, as well as their relationship. The model of immune vasculitis of rabbits were established by intravenous injection of bovine serum albumin twice. Experiment was divided into 4 groups： control group, model group, tanshinone Ⅱa-treated group and aspirin-treated group. The platelet count, platelet aggregation of peripheral blood were determined. The levels of serum IL-1β, IL-6, TNF-α were detected by ELISA. The pathological changes of immune vasculitis were analyzed by hematoxylin ＆ eosin staining, elastic fibers staining and electron microscopy. The results showed that the levels of IL-1β and TNF-α in model group were significantly higher than those in normal group （p 〈 0.05 ）, while the level of IL-6 was not significantly different between various groups. The serum level of IL-1β was correlated with platelet number , while serum levels IL-1β and TNF-α were both correlated with the platelet aggregation. The treatment with tanshinone Ⅱa could significantly decrease the serum levels of IL-1β, TNF-α and platelet number, and the efficacy of tanshinone Ⅱa was same as aspirin. The transhinone Ⅱa and aspirin both could alleviate the vessels damage in patients with immune vasculitis. It is concluded that the tanshinone Ⅱa may diminish the inflammation damage of vessels in patients with immune vasculitis through the inhibition of cytokines and platelets.
出处
《中国实验血液学杂志》
CAS
CSCD
2009年第1期188-192,共5页
Journal of Experimental Hematology
基金
成都市卫生局科研项目(编号0603)
