摘要
目的研究中药复方加味四逆散(JWSNS)对皮质酮和谷氨酸致损伤的PC12细胞中cAMP反应元件结合蛋白(CREB)及其磷酸化的影响。方法以200μmol.L-1Cort和50μmol.L-1Glu损伤的PC12细胞作为体外药理学研究模型,制备JWSNS含药血清,采用免疫组织化学法和West-ernblot法检测PC12细胞损伤模型中CREB和磷酸化CREB蛋白的表达。结果200μmol.L-1Cort和50μmol.L-1Glu可导致PC12细胞中CREB和p-CREB表达下降(CREB阳性细胞率分别为17.1%±4.2%和20.8%±5.7%,p-CREB表达量分别为0.587±0.123和0.515±0.157,P<0.05或P<0.01),10%JWSNS含药血清能升高CREB和p-CREB的表达(CREB阳性细胞率分别为62.6%±11.7%和79.5%±7.6%,p-CREB表达量分别为1.298±0.112和1.269±0.128,P<0.05或P<0.01)。结论10%JWSNS含药血清能通过调节cAMP-CREB信号通路发挥其神经保护和抗抑郁的作用。
Aim To study the effect of Chinese prescription-Jiaweisinisan （JWSNS）on cAMP response element binding protein （ CREB ） and phosphorylation in PC12 cells injured by Corticosterone （Cort） and Glutamate （Glu）. Method 200 μmol · L^-1 Cort and 50 μmol · L^-1 Glu were used to injure PC12 cells which were taken as the pharmacological models in vitro. JWSNS drug-containing serum was prepared and immunohistochemical and Western blot methods were adopted to detect the expressions of CREB and p-CREB in PC12 cell models. Results 200 μmol · L^-1 Cort and 50 μmol · L^-1 Glu could decrease the expressions of CREB and p-CREB in PC12 cells significantly （the CREB positive cell rate was 17. 1% ± 4.2% and 20. 8% ± 5.7% respectively, the expression of p- CREB was O. 587 ±0. 123 and 0. 515 ±0. 157 respectively） （P 〈 0.05 or P 〈 0.01 ）, while 10% JWSNS drug-containing serum increased those significantly （ the CREB positive cell rate was 62.6% ± 11.7% and 79.5% ± 7.6% respectively, the expression of p-CREB was 1. 298 ± 0. 112 and 1. 269 ± 0. 128 respectively） （P 〈0.05 or P 〈0.01）. Conclusion JWSNS can protect nerve and anti-depression by regulating cAMP-CREB signal pathway.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第2期270-274,共5页
Chinese Pharmacological Bulletin
基金
教育部新世纪优秀人才支持计划资助项目(NCET-05-0750)
国家自然科学基金资助项目(No30472256,No30500660)
