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正交实验优选羊蹄中总鞣质的提取工艺研究 被引量:5

Study of Extraction Process of Total Tannin of Rumex Japonicus Houtt L.by Orthogonal Test
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摘要 目的优选中药羊蹄的总鞣质最佳提取工艺。方法采用正交实验L9(34)设计该实验,以总鞣质的含量作为考察指标,用比色法对羊蹄总鞣质的提取工艺进行优选。结果优选工艺为选用8倍药材量的70%乙醇,回流提取1.5h。结论优选工艺提取的总鞣质的含量较高,该工艺合理可行。 Objective To optimize the extraction process of total tannin of Rumex japonicus Houtt L. Methods The optimization of extraction process of total tannin of Rumex japonicus Houtt L was selected by orthogonal test. The content of total tannin was choosed as the assessment index, and the colormetric assay was used for the measurement. Results The optimum extraction process condition was A3B1C1 ,that is adding six times amount of 70% alcohol,reflexing and extracting for 1.5h. Conclusion The optimum extraction process is reliable with higher extracting ratio of the active components.
出处 《医药论坛杂志》 2009年第2期10-11,共2页 Journal of Medical Forum
关键词 正交实验 总鞣质含量 紫外分光光度法 Orthogonal test Total tannin Ultraviolet
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