摘要
目的:探讨应用常规和强化运动训练对脑缺血再灌注大鼠运动功能以及海马区和梗死灶周围微管相关蛋白-2(MAP-2)表达的影响。方法:采用大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞1h,再灌注第7、14和21天,54只造模成功的大鼠随机分为造模对照组(A组)、常规运动训练组(B组)和强化运动训练组(C组),分别采用姿势反射试验、肢体不对称应用试验和角落试验观察各组大鼠的运动功能。结果:3组实验大鼠的3项行为学测试评分在造模后24h均无明显差异,但在第7、14和21天时则有一定差异,与A组比较,除造模后第21天时B组角落试验评分外,B组和C组3项测试评分在造模后第7天、14d和21天均明显好于A组;与B组比较,除造模后第21天肢体不对称应用试验评分外,C组评分在第14天和21天均明显好于B组。MAP-2的免疫组化结果显示,与A组比较,B组和C组在海马区和梗死灶周围MAP-2表达的光密度值在第14天和21天时均明显高于A组,而且C组MAP-2表达的光密度值在第14天和21天时也明显高于B组。结论:运动训练可促进脑缺血再灌注大鼠运动功能的恢复,其机制可能与脑内MAP-2水平上调有关,强化运动训练的效果更明显。
Objective：To explore the effects of conventional and intensive exercise training on motor function and the expression of MAP-2 in the hippocampus and around the cerebral infarcted area of rats after cerebral ischemia-reperfusion. Method：The middle cerebral artery occlusion （MCAO） models was used.The middle cerebral arteries（MCA） of rats were occluded for 1 hour, then reperfused for 7,14 and 21 days. Fifty-four MCAO model-rats were randomized into a control group （Group A）, a conventional exercise training group （Group B） and an intensive exercise training group （Group C）. Neurologic functional behavior tests （postural reflex test, limb asymmetrical usage test and corner test） were performed to test motor function. Result： Except on the 24h after cerebral ischemiareperfusion, there were some differences of neurological function behavior tests＇ scores among the three groups on the 7th, 14th and 21th day. The scores of Group B and C, except the score of corner test of Group B on the 21th day, were significant better than that of Group A on the 7th, 14th and 21th day. The scores of Group C, except the score of limb asymmetrical usage test of Group C on the 21th day, were significant better than that of Group B on the 14th and 21th day. In the hippocampus and around the cerebral infarcted area of rats, significant increase of MAP-2 immunoreactivity were detected in Group B and C, compared with that of group A on the 14th day and 21th day. Significant increase of MAP-2 immunoreactivity of Group C were detected on the 14th and 21th day, compared with that of Group B. Conclusion：Exercise training can promote motor functional recovery. The function enhancement may be partially due to the upregulation of MAP-2 and GFAP. Intensive exercise training is more effective.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2009年第2期99-102,I0001,共5页
Chinese Journal of Rehabilitation Medicine
关键词
缺血再灌注
运动训练
微管相关蛋白-2
cerebral ischemia-reperfusion
exercise training
microtubule associated protein-2
