摘要
本研究以PD培养液为测定培养基,对一株铰链孢属(Alternaria spp.)真菌的培养条件进行了初步研究,通过正交试验设计,确定其最佳培养条件为26℃,160 r/min,装液量120 mL/250 mL,培养72 h时菌丝干重量最高。使用超声波破碎法,从所培养的铰链孢属真菌中提取到分子量为42～43kD的激活蛋白。
The fermentation conditions of Ahernaria spp. were determined through quadrate experiment. The optimum fermentation parameters were as follows： culture temperature 26℃, 120mL liquid medium in 250mL flake, 160r/min for 72 h. Plant activator protein from Ahernaria spp. were extracted using ultrasonic and the molecular weight is 42KD with SDS-PAGE.
出处
《吉林农业科学》
CSCD
2009年第1期17-19,共3页
Journal of Jilin Agricultural Sciences
关键词
铰链孢属真菌
激活蛋白
培养条件
A lternaria spp.
Plant activator protein
Cultural condition
