摘要
目的探讨细胞凋亡及Bcl-2蛋白表达与神经母细胞瘤(NB)发生发展和预后的关系。方法应用SAB免疫组化及TUNEL技术对17例NB中Bcl-2蛋白表达及细胞凋亡进行检测。结果年龄<1岁、Ⅰ～Ⅱ及Ⅳ S期、F型NB凋亡指数(AI)显著高于年龄>1岁,Ⅲ～Ⅳ期及UF型。AI与BFI呈负相关。结论 BEI与AI可作为判断NB生物学特性和预后的重要指标。
Objective To investigate the relationship between the expression of Bcl-2 protein and apoptosis with genessis, development and prognosis of neuroblastomas (NB). Method The expression of apoptosis- suppressing protein Bcl-2 was examined in 17 patients with NB by the standard streptavidin-biotin im- munoperoxidase. Apoptosis was detected in these patients with deoxynucleotidyl transferase-mediated dUTP nick end labeling method. Results Apoptotic index (AI) was significantly higher in the cases (less than one year old) of stage Ⅰ or Ⅱ or Ⅳ S with favorable (F) histopathologic type than that in the cas- es (over one year of age) of stage Ⅲ or Ⅳ with unfavorabie (UF) histopathologic type. There was a negative correlation between expression index of Bcl-2 (BED and AI. Conclusion AI and BEI might be important indicators for predicting biological behavior and prognosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第1期91-92,共2页
Chinese Journal of Experimental Surgery
