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表面活性剂对纤维素酶水解过程的影响 被引量:13

Effects of the Use of Surface Active Agents on the Hydrolysis of Cellulase
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摘要 分别以湿氧化、稀酸常压水解和蒸汽爆破预处理麦草为底物,考察了不同表面活性剂及用量对纤维素酶解过程的影响。实验表明,添加表面活性剂对纤维素酶水解过程有明显影响;添加非离子型表面活性剂(PEG6000、吐温80)可以提高纤维素的可溶性、可发酵糖的转化水平,其中向湿氧化处理的麦草中加入PEG6000后转化水平提高得最大;而添加离子型表面活性剂对酶解起抑制作用。非离子型表面活性剂和纤维素水解底物的最佳比例大约是0.05g/gDM。 Wheat straw pretreated by wet-oxidation, dilute-acid atmospheric hydrolysis and steam explosion respectively were used as substrate, and the effects of the use of different surface active agents at different use levels on the hydrolysis of lignocellulose were investigated. The experimental results showed that the addition of non-ionic surfactants (PEG6000 and Tween80) could increase the conversion of cellulose into soluble and fermentable sugars (the highest conversion level for wheat straw pretreated by wet-oxidation), and the addition of ionic surfactants had the opposite effects. The optimum ratio between non-ionic surfactant and substrate was approximately 0.05 g (g DM)^-1.
出处 《酿酒科技》 北大核心 2008年第12期38-40,43,共4页 Liquor-Making Science & Technology
关键词 木质纤维素 麦草 酶水解 表面活性剂 吸附 lignocellulose wheat straw enzyme hydrolysis surfactant absorption
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