摘要
以边沁桉Eucalyptus benthamii茎段的顶芽或腋芽为外植体开展了组织培养技术的研究，成功建立了边沁桉离体再生体系，为该物种进一步的快速繁殖提供了理论基础．结果表明：初接种芽诱导培养基以MS＋BA0．2mg·L^-1最佳，诱导率为55．8％；最佳继代培养基为MS＋BA0．5mg·L^-1＋IBA1．0mg·L^-1＋NAA0．2mg·L^-1，芽增殖系数为4．6，平均苗高为3．1cm；最佳生根培养基为1／4MS＋IBA1．5mg·L^-1，生根率达93．3％，平均每株生根数为3．2条；试管苗练苗3～7d．移栽到25％糠壳灰＋75％黄心土的基质，移栽成活率可达70％．
Using apical or axillary buds as explants, this paper conducted a study of Eucalyptus bentharnii tissue culture, establishing its in vitro regenerative system to quicken its proliferation. Results show that the optimum medium for initial-shoot induction is MS＋ BA 0.2 mg · L^-1 with an induction rate of 55.8% that the optimum medium for subculture is MS＋BA 0.5 mg· L^-1＋IBA 1.0 mg· L^-1＋NAA 0. 2 mg · L^-1 with a propagation coefficient of 4.6 and an average shoot height of 3.1 cm; and that the optimum medium for rooting is 1/4MS＋IBA 1.5 mg · L ^-1 whose induction rate is up to 93.3% and whose averagenumber of roots is 3.2. Transplanted to pot soil mixed with bran ash and yellow subsoil （1 ： 3） after hardening for 3-7 days, the cultured plantlets enjoyed a survival rate of 70%.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2008年第6期81-85,共5页
Journal of Central South University of Forestry & Technology
基金
科技部国家农业成果转化资金项目(02EFN214300454)
关键词
生物技术
边沁桉
组织培养
培养基
biotechnology
Eucalyptus benthamii
tissue culture
medium
