摘要
[目的]探讨体外实验中纳米氧化硅和微米二氧化硅（SiO2）致人红细胞溶血及脂质过氧化作用的变化情况。[方法]将20nm和60nm两种粒径的纳米氧化硅及微米SiO2粒子悬液（质量浓度均分别为0、0．010、0．020、0．050、0．075、0．100、0．150、0．200、0．400、1．000、1．500、2．000g／L）与正常人红细胞悬液孵育，测定溶血率；两种粒径的纳米氧化硅（质量浓度为0．010、0．020、0．050、0．075、0．100g／L）及微米SiO2（质量浓度为0．200、0．400、1．000、1．500、2．000g／L）悬液分别与正常人红细胞悬液孵育后，测定溶血率及上清液中脂质过氧化产物丙二醛（MDA，硫代巴比妥酸法）含量。[结果]20nm和60nm氧化硅致红细胞溶血率先均随浓度增加而升高，分别在浓度均为0．100g/L时达到100.3％、97．7％的峰值后逐渐下降。微米SiO2粉尘随浓度升高，红细胞溶血率逐渐增加，达2g/L时达峰值93．6％。在0．010～1．000g／L浓度范围内，相同浓度的20nm和60nm氧化硅致红细胞溶血率较微米SiO2高（P〈0．01）；在一定浓度范围内，两种粒径纳米氧化硅及微米SiO2致红细胞溶血率及MDA生成量随其浓度的增加逐渐升高，呈明显剂量-反应关系（P〈0．05）。相同浓度的20nm氧化硅致红细胞溶血率及MDA的生成量较60nm氧化硅高（P〈0．05）。[结论]纳米氧化硅与微米SiO2一样，可诱导红细胞溶血，溶血可能与氧化损伤有关；两种粒径纳米氧化硅致红细胞溶血作用较微米SiO2的作用强，纳米氧化硅对红细胞的毒性作用有尺寸依赖效应。
[ Objective ] To explore the varieties of hemolysis and malondialdehyde （ MDA ）after nanosized silica and microsized silicon dioxide exposure to human normoblast in vitro. [Methods ] Two sizes（ 20hm, 60nm ）of nanosized silica and mierosized silicon dioxide（ final concentrations were 0, 0.010, 0.020, 0.050, 0.075, 0.100, 0.150, 0.200, 0.400, 1.000, 1.500, and 2.000 g/L respectively ）were incubated with human erythroeytes. The hemolytic rates and MDA （ thibabiturie acid assay ）levels in euhure media were measured. [ Results] The hemolytic rates induced by 20 nm and 60 nm nanosized silica and mierosized silicon dioxide elevated along with concentration increase of the three kinds of particles, and reached their peak values of 100.3% 97.7%, 93.6% at levels of 0.100, 0.100, 2.000g/L respectively. Then the hemolytic rates descended as concentration increase of particles. In the 0.010 -1.000 g/L dosage range, the hemolytic rates resulted from two kinds of nanosized silica were higher than that from microsized silicon dioxide at the same concentration （ P 〈 0.01 ）. When erythrocytes were exposed to the three kinds of powder within a dose range, the hemolytic rates and level of MDA increased as the dose increasing with a relationship of doseresponse（ P〈0.05 ）. The hemolytic rates and MDA levels in 20nm nanosized silica treated groups were significantly higher than those of 60 nm nanosized silica under the same dose levels （ P〈 0.05 ）. [ Conclusion ] The results indicated that nanosized silica was the same as mierosized silicon dioxede resulting in hemolysis of erythrocytes. Hemolysis of erythrocytes might be related with effect of oxidative injury. The hemolysis effect on erythrocytes caused by nanosized silica dioxide was stronger than that by microsized silicon dioxide. There was size-dependent effect of nanosized silica on erythroeytes toxicity.
出处
《环境与职业医学》
CAS
北大核心
2009年第1期53-56,共4页
Journal of Environmental and Occupational Medicine
基金
国家重点基础研究发展计划973项目(编号:2006CB705602)
关键词
纳米氧化硅
微米二氧化硅
溶血作用
丙二醛
nanosized silica
microsized silicon dioxide
hemolysis
malondialdehyde
