摘要
以膜生物反应器(MBR)的活性污泥为对象,研究了高速离心法、热提取法、超声波法对活性污泥胞外聚合物(EPS)的提取效果,并确定了三种方法的最优提取条件。结果表明,高速离心法(在16 000 r/min下作用40 min)对EPS的提取率低,对细胞破坏程度小,适合作为一种对照方法来判断其他提取方法对细胞的破坏程度;超声波法(在频率为40 kHz、功率为50 W下提取20 min)对EPS提取不完全,可以与其他方法结合作为一种有效的预处理手段;而热提法(在80℃下提取20 min)对EPS的提取率虽然较高,但对EPS及细胞体破坏程度较大,不适合对提取的EPS作进一步的成分分析。
The extraction of extracellular polymeric substances （EPS） membrane bioreactor （MBR） was studied by three physical means： ultracen activated sludge in the trifugation, thermal and ultrasonication methods. The optimal extraction conditions were determined. The ultracentrifugation with 16 000 r/min and reaction time 40 minutes has low extraction efficiency of EPS but little cell disruption. It can serve as a control method to estimate the cell disruption caused by other methods. The uhrasonication with frequency 40 kHz, power 50 W and reaction time 20 minutes has no complete EPS extraction. It can be used as a preliminary treatment in conjunction with another extraction method. The thermal extraction, under temperature 80 ~C and reaction time 20 minutes, is effective, but causes great EPS and cell disruption. The extracted EPS is not fit for farther composition analysis.
出处
《中国给水排水》
CAS
CSCD
北大核心
2009年第5期80-83,88,共5页
China Water & Wastewater
基金
天津市应用基础研究计划项目(07JCZDJC02100)
关键词
膜生物反应器
物理提取
胞外聚合物
membrane bioreactor （MBR）
physical extraction
extracellular polymeric substances （EPS）
