摘要
建立了以索氏抽提,凝胶渗透色谱及硅胶-氧化铝复合层析柱净化,测定蔬菜中13种多溴联苯醚(PBDEs)的GC-NCIMS分析方法,方法的检出限以干重计为5.4～15.7pg/g,加标样品的回收率和RSD分别为78%～97%和0.6%～10.0%。还研究了蔬菜中的PBDEs在人胃肠中对人体的生物有效性,胡萝卜和菠菜中PBDEs的总含量分别为424.1pg/g和981.3pg/g,对人体的平均生物有效性分别为33.5%和2.6%,计算得到13种PBDEs在胡萝卜和菠菜中的生物有效含量分别为132.6pg/g和26.1pg/g,研究结果对污染物的暴露风险评估有参考价值。
A method for 13 polybrominated diphenyl ether（PBDEs） detection in vegetable was developed. The samples were treated by Soxhlet extraction, and cleaned up by gel permeation chromatog- raphy column and multilayer silica - alumina column. The detection of analytes was performed by gas chromatography mass spectrometry negative ion chemical ionization detection （ GC - NCI MS）. The results showed that the detection limits of 13 tri- to hepta-BDEs were in the range of 5.4 - 15.7 pg/g dry weight. The recoveries were between 78% and 97% with RSD of 0.6% - 10. 0%. In addition, the bioaccessibility of PBDEs in the simulating human gastrointestinal tract were investigated. The concentrations of PBDEs in carrot and spinach were 424. 1 pg/g and 981.3 pg/g, and the average bioaccessibility were 33.5% and 2.6% , respectively. The total bioaccessible concentrations of PB- DEs in carrot and spinach were i32.6 pg/g and 26. 1 pg/g, respectively. The obtained results may provide useful information for pollutant exposure assessment to human.
出处
《分析测试学报》
CAS
CSCD
北大核心
2009年第1期1-6,共6页
Journal of Instrumental Analysis
基金
国家重点基础研究规划项目(2008CB418205)
国家重点实验室开放基金资助项目(OGL-200704)
关键词
多溴联苯醚
蔬菜
体外实验
生物有效性
polybrominated diphenyl ether（PBDEs）
vegetable
in vitro test
bioaccessibility
