摘要
[目的]探讨不同培养基配方诱导蝴蝶兰原球茎的效果及其诱导因素。[方法]以蝴蝶兰(f001和f006)试管苗根尖和叶为外植体,以MS和KC为基本培养基,分别添加不同浓度的6-BA、NAA和活性炭,各设置16个处理组进行原球茎诱导,研究其对诱导蝴蝶兰原球茎效果的影响。[结果]在KC+6-BA 1.0 mg/L+NAA 0.3 mg/L+AC 0.3%的培养基中,蝴蝶兰f006根段原球茎状体的诱导率可达20%;f001根段的诱导培养基配方采用KC+6-BA 1.0 mg/L+NAA 0.2 mg/L+AC 0.2%,其原球茎的诱导率达17%,f001叶片的诱导培养基配方采用KC+6-BA 4.0 mg/L+NAA 0.2 mg/L+AC 0.1%,其原球茎的诱导率达10%。[结论]该研究中所用的6-BA和NAA激素和活性炭浓度在MS和KC培养基上对蝴蝶兰原球茎的诱导影响均不显著;不同外植体在不同培养基中其适宜的外源激素浓度与组合也不同。
[Objective] The study aimed to discuss the effects of different media formulas for inducing Phalaenopsis protocorm and their inducing factors. [Method] With the root tip and leaf of Pbalaenopsis（fOOland fO06） test-tube shoots as explants,the MS and KC were taken as the basic medium adding different concn.of 6-BA and NAA and activated carbon resp.to set up the 16 treatments for protocorm induction and their effect on inducing Phalaenopsis protocorm were studied. [ Result] In the medium of KC ＋ 6-BA 1.0 mg/L ＋ NAA 0.3 mg/L ＋ AC 0.3%, the protocorm induction rate of Pha/aenops/s f006 root segment was 20 %. When the induction mediums for f001 root segment was K C ＋ 6-BA 1.0 mg/L ＋ N AA 0.2 mg/L ＋ A C 0.2 %, the protocorm induction rate was 17% .When the induction mediums for leaves was KC ＋6-BA 4.0 mg/L ＋ NAA 0.2 mg/L ＋ AC 0.1% ,the protoconn induction rate was 10%. [Conclusion] There was no significant effect of 6-BA and NAA hormones and the activated carbon concn, on the induction of Phalaenopsis proto- corm on MS and KC medium. The different explants on different medium had different exogenous hormone concn, and matching.
出处
《安徽农业科学》
CAS
北大核心
2009年第3期976-978,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30660091)
云南省应用基础研究计划项目(2006C0084M
2006C0086M)
云南省中青年学术技术带头人后备人才培养计划项目(2006PY01-61)
楚雄州学术技术带头人项目(200401)
楚雄师范学院重点学科建设项目(05YJJSXK03)
楚雄师范学院学术带头人专项项目(05Y-JDTR08)
关键词
蝴蝶兰
根段
叶片
原球茎
诱导
Phalaenopsis
Root segment
leaf
Protocorm
Induction
