摘要
利用家兔LRP蛋白聚集形成蛋白复合物的性质,首先通过两次蔗糖密度梯度离心从家兔肝脏中分离得到LRP粗提液,再利用分子大小差异,使用Sephacryl-1000分子筛层析纯化,SDS-PAGE银染结果显示最终得到了单一电泳条带的家兔LRP蛋白。最后使用LRP单克隆抗体的Western blotting鉴定结果正确。
In the case of LRP protein assembling into a protein complex, two steps of sucrose density gradient centrifugation were used to separate the LRP protein from rabbit livers. Then followed by the Sephacryl-1000 gel filtration, and after the Ag＋ dyeing for SDS-PAGE gel, a perfect single band was shown. The Western blotting using the LRP monoclone antibody was shown a correct result.
出处
《四川动物》
CSCD
北大核心
2009年第2期256-258,共3页
Sichuan Journal of Zoology
