摘要
为了制备高效微生物膜,从污水中筛选菌种(QT2061),经B IOLOG鉴定为枯草芽孢杆菌。并以聚乙烯醇为包埋剂制作BOD生物传感器微生物膜,通过对国家标准样品及几种典型水样的检测,结果表明,该微生物传感器的响应时间为8 min,可连续稳定测试10 d以上;对国家标准溶液的测试结果准确度较高,相对误差≤5%;对实验中几种水样均有响应,测试结果与5日法的测试结果有良好的相关性(相对偏差≤5%)。
In order to get some efficient microbial membrane, a Bacillus subtilis A （QT2061） was selected from wastewater, and was identified by the MicroStation System. It was used as the sensitive material for the BOD sensor（BODS） after being immobilized by PVA. Some practical wastewater samples and national standard BOD solutions were measured with the BODS compared with the traditional 5-day method. The result showed that the response time of the BOD sensor was only 8 rain, furthermore, BODS could be kept stable for up to 10 days without significant loss in their activity. BOD in standard BOD solutions were estimated using the BODS with deviation within 5%. The BODS was also omnivorous to the real samples with a generally good agreement between the BOD values estimated by the biosensor and the conventional 5-day test.
出处
《环境工程学报》
CAS
CSCD
北大核心
2009年第3期437-441,共5页
Chinese Journal of Environmental Engineering
基金
国家建设部项目(05-K2-6)
青岛理工大学博士基金资助项目(C2007-006)
