摘要
目的:观察牙齿缺失对小鼠空间学习记忆能力的影响。方法:将健康成年小鼠100只随机分成1、3、6个磨牙缺失组、麻醉对照、正常对照5组。在麻醉和拔牙手术后1周开始,采用Morris水迷宫系统测试各组小鼠的学习记忆能力,每周测试1次,连续测试6周。结果:在历时6周的行为学测试中,1～4周的小鼠游泳训练平均逃避潜伏期显著高于5、6周(P<0.05),潜伏期有下降趋势;6个磨牙缺失组小鼠的游泳训练平均逃避潜伏期显著高于其它4组(P<0.05);雌雄小鼠同一时期的潜伏期,以及麻醉对照组与正常对照组的潜伏期间均不具有显著差异性(P>0.05)。结论:多数目牙齿缺失对小鼠空间学习记忆能力会造成一定损伤,而个别牙齿缺失对小鼠空间学习记忆能力则无明显影响。
AIM： To investigate the effect of missing molars on spatial learning and memory in mice and explore its mechanism. METHODS： 100 healthy and adult mice were randomly divided into the following five groups, of which 1,3 and 6 molars were missing in three groups respectively, while no molars were missing in the anesthetic and control groups. The ability of spatial leaming and memory of these mice was tested with the Morris water maze 1 week after anesthesia and operation of tooth extraction. The experiment lasted for six weeks with once per week. RESULTS ： During the six weeks for behavior tests, the performances of spatial learning and memory were significantly improved at 5 and 6 weeks in comparing with that in previous four weeks （ P 〈 0.05 ）. The latent period for targeting the platform for mice in the group of 6 molars missing was obviously longer than other groups （ P 〈 0.05 ）, while there was no conspicuous difference among other groups. No gender difference was observed. CONCLUSION： A relatively large number of missing molars will cause a certain effects on the ability of spatial learning and memory of the mice, while no effect could be seen on the mice missed fewer molars.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2009年第2期77-80,93,共5页
Chinese Journal of Conservative Dentistry
基金
2006年教育部科学技术研究重点项目(106152)
2007年甘肃省科技支撑项目(0708NKCA109)
