摘要
建立了反相高效液相色谱同时测定膏霜类化妆品中7种氟喹诺酮类药物（诺氟沙星、培氟沙星、环丙沙星、丹诺沙星、恩诺沙星、沙拉沙星、双氟沙星）的方法。样品先用乙腈-水（0.1%甲酸）（体积比5∶95）溶液提取,经正己烷脱脂净化后用反相高效液相色谱法测定。添加水平为1.0～10.0 mg/kg时,回收率为87%～108%,相对标准偏差为2.3%～5.8%。结果表明,该法操作简便、灵敏、准确,适用于膏霜类化妆品中氟喹诺酮类药物的测定。
A reversed-phase high performance liquid chromatography（RP-HPLC） method was established for the determination of seven fluoroquinolones, such as norfloxacin, pefloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin and difloxacin, in moisturizers and creams cosmetics. Cosmetic sample was extracted with acetonitrile-0. 1% formic acid（5 ： 95, by volume）, and then the extract was quantified by reverse phase high performance liquid chromatography after it was purified by hexane. The calibration curves showed good linearity in the range of 0.5 - 5.0 mg ·L^-1 for pefloxacin, ciprofloxacin, and difloxacin, 0.2 -5.0 mg ·L^-1 for the rest of analytes. The recoveries of seven fluoroquinolones from spiked samples at three concentration levels of 1, 5, 10 mg/kg were ranged from 87% to 108% with RSDs of 2. 3% -5.8%. The results indicated that the method was simple, sensitive and accurate, and was suitable for the determination of fluoroquinolones in cosmetic sample.
出处
《分析测试学报》
CAS
CSCD
北大核心
2009年第2期235-238,共4页
Journal of Instrumental Analysis
基金
国家质检总局科技计划资助项目(2007QK302)
广东省科技计划资助项目(2008B080600007)
关键词
反相高效液相色谱法
氟喹诺酮类
化妆品
reversed-phase high performance liquid chromatography （ RP-HPLC ）
fluoroquinolones
cosmetics
