摘要
【目的】确定导致大鲵(Andrias davidiamus,giant salamander)腹水病的主要病原菌类型。【方法】从患病大鲵的腹水和肠道分离细菌并进行纯培养,用细菌自动鉴定系统和分子生物学方法对培养细菌进行鉴定,再对52尾健康的1龄大鲵进行感染试验,以确定病原菌,同时对病原菌进行药物敏感试验。【结果】经过细菌自动鉴定系统确定了4株细菌,室内感染试验确定嗜水气单胞菌(Aeromonas hydrophila)为主要致病菌;细菌16S rDNA基因序列分析也再次证明该菌为嗜水气单胞菌;药物敏感试验显示,该病原菌对庆大霉素、环丙沙星、阿米卡星等药物敏感,对氨卞青霉素、舒巴坦、头孢唑啉等药物具有抗性。【结论】导致大鲵腹水病的主要病原菌为嗜水气单胞菌。
[Objective] The research identified the pathogenic becteria of giant salamander （Andrias davidiamus） that suffered from ascites disease. [Method] Becteria were isolated from the intestine and as- cites of two freshly died giant salamanders. Fifty two Giant salaman^lers were used to make experimental infection with injection baeterial suspension by intraperitoneal injection. The becteria were identified by the Gram＇s staining,VITEK Bacterial Automatic Identification System and sequence analysis of 16S rDNA gene. [Result] Four different becteria were isolated and finally the becterium 2 # FS,which was identified as Aeromonas hydrophila ,copied the typical diseased symptoms naturally. So the Aerornonas hydrophila was the main pathogen of ascitesosis disease pathogen of giant salamander. Sequence analysis of 16S rDNA showed that the main pathogen was Aeromonas hydrophila too. The susceptibility test to antibiotics re- vealed the isolated pathogen was resistant to Ampicillin etc and sensitive to Ciprofloxacin, etc. [Conclusion] Aerornonas hydrophila was the pathogenic becteria resulted in ascites disease of gaint salamander.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第3期77-81,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
农业科技成果转化项目(05EFN216900350)
国家科技支撑计划专项项目(2006BAD03B08-04)
国家科技基础条件平台专项项目(2006DKA30470-002)
中央级公益性科研院所基本科研业务费专项资金项目
关键词
大鲵
腹水病
嗜水气单胞菌
giant salamander（Andrias davidiarnus ）
Ascitesosis disease
Aerornonas hydrophila
