摘要
本研究以抗黄瓜花叶病毒病的烟草品种铁把子和台烟8号,感病品种NC82和中烟15为亲本,构建F1、F2、BC1代分离群体,并对它们进行CMV的接种鉴定和抗性遗传分析,结果表明铁把子和台烟8号对CMV的抗性是由1对隐性基因控制的。依据混合群体分组分析法(BSA),从F2代群体植株中选取DNA建立黄瓜花叶病毒病的抗病基因池和感病基因池,利用135对SSR引物进行分子标记和分析,得到标记SM1350与来源于铁把子的CMV抗性基因间的遗传距离为8.64cM,标记SM2270与来源于台烟8号的CMV抗性基因间的遗传距离为3.92cM。
The study was carded out using F1, F2, BC1 populations from a cross between CMV resistant varieties tiebazi/taiyan-8 and CMV susceptible varieties NC82/zhongyan15. They were identified by artificial inoculation on seedlings in greenhouse and the inheritance of resistance were analyzed. Results showed that the resistant character of both resistant parents is controlled by single recessive gene. DNAs of F2 population were extracted to develop into a resistant pool and a sensitive pool based on the BSA （bulk segregant analysis）. 135 pairs SSR primers were used for molecular marker analysis. The results showed that the genetic distance between SM1350 and resistant genes from ＂tiebazi＂ is 8.64 cM. And the genetic distance between SM2270 and resistant genes from ＂taiyan-8＂ is 3.92 cM.
出处
《分子植物育种》
CAS
CSCD
2009年第2期355-359,共5页
Molecular Plant Breeding
基金
国家烟草专卖局科技项目(110200601008)
院所基金基本科研业务费专项(082060302-09)资助
关键词
烟草
黄瓜花叶病毒
SSR
抗性遗传分析
Tobacco, Cucumber mosaic virus, SSR, Genetic analysis of resistance
