摘要
目的观察转录因子T-bet和GATA-3在食管癌患者外周血单个核细胞（PBMC）中的表达，并探讨其与患者免疫状态的关系。方法采用逆转录-荧光定量-聚合酶链反应（RT-FQ-PCR）检测60例食管癌患者和30例健康对照者PBMC中T-bet和GATA-3的mRNA表达水平，采用酶联免疫吸附法（ELISA法）检测血浆中干扰素γ（IFN-γ）和白细胞介素4（IL-4）的水平。结果与健康对照组相比，食管癌患者T-bet mRNA表达明显降低（Ⅰ、Ⅱ期食管癌组为0．27±0．05，Ⅲ、Ⅳ期食管癌组为0．12±0．02），GATA-3 mRNA表达明显升高（Ⅰ、Ⅱ期食管癌组为0．45±0．06，Ⅲ、Ⅳ期食管癌组为0．55±0．03），IFN-γ表达明显降低[Ⅰ、Ⅱ期食管癌组为（12．12±1．48）ng／L，Ⅲ、Ⅳ期食管癌组为（8．44±0．90）ng／L]，IL-4表达明显升高[Ⅰ、Ⅱ期食管癌组为（18．64±0．77）ng／L，Ⅲ、Ⅳ期食管癌组为（25．28±2．02）ng／L]，Ⅰ、Ⅱ期食管癌组与Ⅲ、Ⅳ期食管癌组间差异无统计学意义（均P〉0．05）。IFN-γ水平与T-bet mRNA表达呈正相关（r=0．79，P〈0．05），IL-4与GATA3 mRNA表达呈正相关（r=0．43，P〈0．05）。结论食管癌患者外周血中T-bet表达降低，GATA-3表达升高，Th1和Th2平衡被打破，Th2占优势。T-bet和GATA-3参与了Th1和Th2平衡的调控。
Objective To investigate the expression of transcription factors （TF） T-bet and GATA-3 mRNA in peripheral blood mononuclear cells and its correlation with immune status in esophageal cancer patients. Methods Sixty patients were divided into two groups according to the clinical data： group A consisting of stage Ⅰ and Ⅱ, group B including stage Ⅲ and Ⅳ. The gene expression of T-bet and GATA-3 in 60 esophageal cancer patients and 30 healthy controls was detected by reverse transcription-polymerase chain reaction （RT-PCR）. The expression of IFN-γ and IL-4 was measured by enzyme linked immunosorbent assay （ ELISA）. Results Expression of T-bet mRNA in esophageal cancer patients（ stage Ⅰ and Ⅱ： 0. 27 ±0.05 ng/L, stage Ⅲ and Ⅳ ： 0.12 ±0.02 ng/L）was significantly lower than that in the healthy controls （ 1.35 ± 0.14 ng/L） , but the expression of GATA-3 mRNA in esophageal cancer patients （ stage Ⅰ and Ⅱ ： 0.45 ± 0.06, stage Ⅲ and Ⅳ ： 0.55 ± 0.03 ） was significantly higher than that in the healthy controls （ 0.09 ± 0.10）. The plasma level of Th1 cytokine IEN-γ in the patients [ stage Ⅰ and Ⅱ： （12.12 ± 1.48） ng/L, stage Ⅲ and Ⅳ：（8.44 ± 0.90） ng/L] was significantly lower than that in the healthy controls, while the level of Th2 cytokine IL-4 in the patients [ stage Ⅰ and Ⅱ ： （18.64 ±0.77） ng/L, stage Ⅲ and Ⅳ： （25.28± 2.02） ng/L] was significantly higher than that in the healthy controls. However, neither in the expression of T-bet and GATA-3, nor in the plasma level of IFN-γ and IL-4, showed a significant difference between group A and B. Conclusion In the peripheral blood of esophageal cancer patients, the expression of T-bet decreased, while GATA-3 increased, Th1/Th2 balance is broken, and the Th2 is dominant. T-bet and GATA-3 play a part role in the regulation of Th1/Th2 balance.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2009年第3期189-191,共3页
Chinese Journal of Oncology
