摘要
目的探讨低分子肝素(LMWH)对胃癌SGC-7901细胞增殖及其人端粒酶逆转录酶(hTERT)表达的影响。方法不同浓度LMWH作用于SGC-7901细胞不同时间后,MTT法检测细胞生长抑制率,流式细胞仪分析细胞周期,RT-PCR检测细胞hTERT mRNA。结果LMWH作用后,SGC-7901细胞的生长受抑制,呈剂量和时间依赖性,细胞停滞于G1期,其hTERT mRNA表达下降。结论LMWH可抑制SGC-7901细胞的生长,其机制可能与阻滞细胞周期、降低hTERT mRNA的表达有关。
Objective To explore the effect of low molectdar weight heparin（LMWH） on cell proliferation and the expression of hTERT in gastric carcinoma SGC-7901 cell line. Methods SGC-7901 cells were treated with different concentration of LMWH for different time. The inhibitory rate of SGC-7901 cells were evaluated by MTT. The cell cycle distributions were analyzed by flow cytometry.The hTERT mRNA in the cells were determined by RT-PCR.Results Cell proliferation was inhibited in time -dependent and dose-dependent, incubation with LMWH arrested cells at GI phase of the cell cycle. In addition, the expression of hTERT- mRNA was reduced. Conclusions LMWH can inhibit the proliferation of SGC-7901 cell, the mechanism may invole in arresting cell circle and reduce the expression of hTERT mRNA.
出处
《山东医药》
CAS
北大核心
2008年第33期25-26,共2页
Shandong Medical Journal
基金
江苏省无锡市科技局社会发展计划基金资助项目(CLZ00612)
关键词
低分子肝素
胃肿瘤
SGC-7901细胞
端粒酶逆转录酶
low molecular weight heparin
stomach neoplasms
SGC-7901 cell
telomerase reverse transcriptase
