摘要
目的:建立固相萃取-高效液相色谱法测定水果中赤霉素、苯甲酸、糖精钠和胭脂红含量的方法。方法:水果样品经50%甲醇-水提取后,用HLB萃取,以甲醇+水(3+1)洗脱,洗脱液经C18柱分离、二极管阵列检测器检测,用高效液相色谱仪测定水果中赤霉素、苯甲酸、糖精钠和胭脂红。结果:HLB萃取柱可有效去除样品中干扰物质,浓缩样品中的被测物质,方法定量准确,线性好,相关系数r>0.999,相对标准偏差<5.0%,加标回收率可达90%以上。结论:本方法萃取效率高,灵敏度高,定量准确,可用于测定水果中赤霉素、苯甲酸、糖精钠和胭脂红。
Objective：To establish a SPE-HPLC method for the determination of gibberellin,benzoic acid,saccharin sodium and ponceau 4R in fruits.Methods：Fruit samples were extracted with methnal/water（50%） and cleaned up with Waters HLB solid extration phase.Then gibberellin,benzoic acid,saccharin sodium and ponceau 4R were determined by HPLC with PAD detector.Results：The four components were concentrated and separated completely by HLB cartridges.The good correlation and coefficient of the standard curve were obtained（r〉0.999）,the recoveries of sample were 92%～104% with relative standard deviations between 3.5% and 4.3%.Conclusion：This method is simple,reliable and accurate for determination of gibberellin,benzioc acid, saccharin sodium and ponceau 4R in fruits.
出处
《中国卫生检验杂志》
CAS
2009年第3期579-581,共3页
Chinese Journal of Health Laboratory Technology
基金
广州市医药卫生科技项目(2007-YB-187)
