摘要
【目的】对甜菜耐氯嘧磺隆生化机理进行研究,为甜菜耐氯嘧磺隆基因型的合理应用提供理论依据。【方法】以耐性甜菜为试验材料,采用田间框栽和水培试验相结合的方法,对甜菜在氯嘧磺隆胁迫下的生化机理进行研究。【结果】土壤中氯嘧磺隆0.5～1.5ga.i.·ha-1剂量下,耐性甜菜品系叶片中GST活性明显增加,根中GSH含量较对照增加50.0～490.1μg·g-1。敏感甜菜在氯嘧磺隆用量为0.5ga.i.·ha-1时,叶片中GST活性略有增加,但较耐性甜菜低122.6U·(min·mgprotein)-1,根中GSH含量与对照相比仅增加7.4μg·g-1。氯嘧磺隆0.5ga.i.·hm-2施用剂量下,耐性甜菜苗期和叶丛形成期ALS活力与对照相比分别增加62.5%和70.6%,而此时敏感甜菜品系ALS活力与对照相比则分别降低36.8%和64.8%。【结论】耐性甜菜GST、GSH活性及氯嘧磺隆作用靶酶ALS活力在氯嘧磺隆施用后短时间内快速升高是甜菜对氯嘧磺隆耐性增强的重要原因。
[Objective] Effect of chlorimuron-ethyl on biochemical mechanism in tolerant sugar beet was investigated to provide basic data on using the tolerant genotype to chlorimuron-ethyl properly. [Method] Tolerant sugar beet was used to analyze its biochemical mechanism under chlorimuron-ethyl stress with fi＇ame culture in field and water culture. [Result] Glutathione S-transferase （GST） activity in leave of tolerant sugar beet was remarkably increased as chlorimuron-ethyl was used at 0.5 ga.i.·ha^-1 and 1.5 ga.i.·ha^-1 field dose, at the same time glutathione （GSH） content increased by 50.0-490.1 μg·g^-1 GST activity of sensitive sugar beet decreased by 122.6 U.（min-mg）^-1 compared with tolerant sugar beet and GSH content only increased by 7.4 μg.g^-1 as chlorimuron-ethyl was at 0.5 ga.i..ha^-1 field dose in sensitive sugar beet. The higher GST activity and GSH content could conjugate chlorimuron-ethyl which had been absorbed in tolerant sugar beet and made it lost activity. Acetolactate synthases （ALS） activity of the tolerant sugar beet increased by 62.5% and 70.6%, respectively, in seedling and leaf growth period, at the same time ALS activity of the sensitive variety decreased by 36.8% and 64.8%, respectively. [Conclusion] The rapid enhancement ofGST, GSH and the target enzyme activity of ALS is the important reason for the tolerant sugar beet enduring to chlorimuron-ethyl.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第3期906-911,共6页
Scientia Agricultura Sinica
基金
黑龙江省教育厅项目(11511039)
黑龙江省博士后基金项目(LBH-Z05222)
东北农业大学博士启动基金项目
