摘要
从河北省不同地区未使用过啶菌噁唑的保护地中采集黄瓜或番茄灰霉病果、病叶,经单孢分离获得102株灰葡萄孢菌,采用菌丝生长速率法测定其对啶菌噁唑的敏感性,所得EC50值在0.04600.1991μg/mL之间,平均为（0.1182±0.0363）μg/mL,其敏感性呈连续单峰曲线分布,可作为灰葡萄孢菌对啶菌噁唑的敏感性基线。采用紫外线诱导获得了7株抗药突变体,抗药突变体的菌落直径、菌丝干重、产孢量和致病性明显低于其亲本菌株,继代培养9代后抗药突变体的抗药性倍数下降。
A total of 102 single-spore isolates were collected from cucumber or tomato infected by Botrytis cinerea without exposure to SYP-Z048 in protected fields in different regions of Hebei Province.The sensitivity of the isolates was determined by mycelial growth rate inhibition tests.The EC50 value distributed as a unimodal curve,which ranging from 0.046 0 μg/mL to 0.199 1 μg/mL,with a mean value of（0.118 2±0.036 3） μg/mL.The data could be used as baseline sensitivity of B.cinerea to SYP-Z048.Seven SYP-Z048-resistant mutants were obtained through ultraviolet radiation.The colonies diameter,mycelial weight,sporulation and pathogenicity ability of resistant mutants were significantly lower than that of parental isolates.The resistance rate of resistant mutants receded after nine incubations.
出处
《农药学学报》
CAS
CSCD
北大核心
2009年第1期141-144,共4页
Chinese Journal of Pesticide Science
基金
国家"十一五"科技支撑计划项目(2006BAD08A03-2)
关键词
灰葡萄孢菌
啶菌噁唑
敏感性基线
抗药突变体
生物学性状
Botrytis cinerea
SYP-Z048
baseline sensitivity
resistant mutants
biological characteristics
