摘要
利用随机扩增多态性技术研究生姜连作地、施加有机肥后的生姜连作地及新开地中土壤微生物多样性与生姜青枯病的关系,结果发现,连作地的青枯病发病率为100%、新开地没有发病,而施加有机肥的连作地生姜青枯病发病率降至37.64%。发现施加有机肥连作地的真菌、放线菌数量增加。多样性指数分析发现Shannon指数、Simpson指数等均显著增加。随机扩增条带出现连作地的条带丰富度较少、新开地的条带最多、施加有机肥连作地的RAPD条带明显多于连作地,接近新开地,并出现了一些连作地没有的特异条带。这些结果表明,施加有机肥可以改善土壤微生物群落结构,提高微生物多样性,从而抑制病害发生。
Relationship between ginger bacterial wilt （GBW） and its soil microbial biodiversity of freshly ploughed soil, continuous cropping ginger field and freshly ploughed one applying organic fertilizer were studied by Random Amplification Polymorphic DNA （RAPD） technique. It was found through the results that GBW did not come on in freshly ploughed soil, while 100% occurred in continuous cropping field. However, the incidence of GBW dropped to 37.64% in continuous cropping field applying organic fertilizer. It was found also the number of fungi, actinomyces increased in the continuous cropping field that applying organic fertilizer. Analysis by diversity index found that Shannon index and Simpson index etc were greatly increased. The abundance of random amplification band appeared a fairly few of amplification DNA bands in continuous cropping soil, while the most in freshly ploughed field; the RAPD bands of continuous cropping field applied with organic fertilizer apparently more than continuous cropping field and close to freshly ploughed field, and appeared some new specific band that was non in the continuous cropping field. All of these indicated that the application of organic fertilizer could improve the structure of soil microbial flora, and increase microbial diversity, thereby to inhibit the coming on of the diseases.
出处
《微生物学杂志》
CAS
CSCD
2009年第1期74-78,共5页
Journal of Microbiology
基金
重庆市科委资助项目(021204)
关键词
生姜青枯病
有机肥
RAPD
微生物多样性
bacterial ginger wilt （BGW）
organic fertilizer
RAPD
microbial diversity
