摘要
目的采用静电纺丝技术制备聚乙二醇纳米纤维膜、聚乳酸纳米纤维膜,通过大鼠腹腔黏连动物模型,对比观察这两种材料预防术后腹腔黏连的作用。方法选用雄性SD大鼠42只,采用盲肠刮伤法制造大鼠腹腔黏连动物模型,随机将动物分为对照组(A组)、聚乙二醇纳米纤维膜组(B组)和聚乳酸纳米纤维膜组(C组)。在关腹前,B组和C组创面分别用聚乙二醇纳米纤维膜与聚乳酸纳米纤维膜覆盖,对照组创面不做处理。于术后2周处死大鼠,行大体、光镜及电镜观察。结果两种纳米纤维膜可以很容易的黏附到损伤的腹膜表面和盲肠浆膜表面,在2周内逐渐被机体降解。术后2周,实验组的黏连分级程度低于对照组,差异有统计学意义(P<0.01),C组黏连的程度和发生率低于B组,差异无统计学意义(P>0.05)。光镜下对照组纤维组织增生及炎性反应程度较实验组活跃。扫描电镜下见实验组创面修复情况优于对照组。结论两种纳米纤维膜均为生物可吸收降解材料,均可降低大鼠术后腹腔黏连的发生率和黏连程度。聚乳酸纳米纤维膜可以引起更轻微的纤维化和炎性反应,其作用优于聚乙二醇纳米纤维膜。
Objective To produce the polyethylene glycol nanofiber membrane and polylactic acid nanofiber membrane by electrospinning technique,and to compare the effects of the two nanofiber membranes on the prevention of postoperative intra-abdominal adhesion.Methods Male Sprague-Dawley rats were used to establish abdominal adhesion model by cecum abrasion method.42 SD rats were randomly divided into three groups,two in the experimental groups and one in control group.During operation,the cecum wounds were covered with polyethylene glycol nanofiber membrane or polylactic acid nanofiber membrane respectively in experimental group A and B,while in the control group,the cecum wound was leave alone,finally,the abdominal incision was closed.Rats were then sacrificed 2 weeks postoperatively.Tissue samples were examined grossly,as well as under light and electron microscope.Results The two nanofiber membranes adhered to damaged peritoneal surface and cecum serosa surface easily,and underwent degradation within 2 weeks gradually.At 2-week point,the degree of adhesion scales in the two experimental groups were less than control group significantly(P < 0.01) ;the incidence and degree of adhesion in experimental group B was lower than that in group A,although the difference was not statistically significant(P > 0.05).The fibrous tissue generation and inflammatory reaction intensity in the control group were much more active than those in the experimental groups under light microscopic observation,while the wound healing process in the experimental groups were better than that in the control group as measured under electron microscope.Conclusions Both these two nanofiber membranes are biodegradable materials,which can reduce the incidence and intensity of postoperative intra-abdominal adhesion in rats.Polylactic acid nanofiber membrane exhibits better antiadhesive effect than polyethylene glycol nanofiber membrane,and associates with less fibrosis and inflammatory responses in rat models.
出处
《中华临床医师杂志（电子版）》
CAS
2012年第23期7625-7629,共5页
Chinese Journal of Clinicians(Electronic Edition)
基金
教育部科学技术研究重点项目(109072)
关键词
黏连
聚乙烯二醇类
聚乳酸
Adhesions
Polyethylene glycols
Polylactic acid
