摘要
目的：探讨外源性透明质酸（ＨＡ）抑制瘢痕形成的作用及其可能机制。方法：健康ＳＤ大鼠４５只，复制背部创伤模型，随机分成２％ＨＡ治疗组（Ａ组）、１％ＨＡ治疗组（Ｂ组）、ＰＢＳ对照组（Ｃ组），观察创面大体形态，平均愈合时间和残留面积的大小；并测定愈合部位组织蛋白、羟脯氨酸（Ｈｙｐ）、Ⅲ型前胶原（ＰＣⅢ）的含量。结果：（１）３组的平均愈合时间为（１５．２±２．０），（１４．２±１．６）和（１３．４±１．４）ｄ，Ａ、Ｂ两组与Ｃ组比较有显著性差异（Ｐ＜０．０５）。Ａ、Ｂ两组的残留面积与Ｃ组比较有显著性差异，３组间比较也有显著性差异（Ｐ＜０．０５）。（２）Ｈｙｐ和ＰＣⅢ含量的测定：Ａ、Ｂ两组Ｈｙｐ含量的均值低于Ｃ组（Ｐ＜０．０１），而ＰＣⅢ的含量却高于Ｃ组（Ｐ＜０．０１）。结论：（１）ＨＡ通过对细胞的调控作用，可有效地抑制胶原的合成，提高Ⅲ型胶原含量，使创伤愈合过程中瘢痕形成减少。
Objective: To evaluate the effects of hyaluronic acid(HA) on the inhibition of scar formation and their possible mechanisms. Methods: Forty-five healthy adult SD rats in a dorsal part trauma model were randomly divided into 2% HA treatedgroup (group A), 1% HA treatedgroup (group B), and PBS treatedgroup as a control group(group C). The process of healing was observed, the remained wound area was measured, and mean wound healing time was calculated. Furthermore, dynamic analysis of local tissue protein, Hyp and PC Ⅲ contents was performed. Results: (1) The remained wound area of control group was much smaller than that in treated group, and the mean value of wound healing time was(15.2±2.0), (14.2±1.6) and (13.4±1.4)d, respectively. (2) The contents of tissue Hyp were statistically lower in HAtreated groups, while those of PC Ⅲ were statistically higher as compared with. Conclusion: (1) The exogenous hyaluronan can effectively inhibit collagen synthesis and elevate tissue PCⅢ content. As a result, the scar formation is decreased. (2)HA efficacy presents a dosedependent manner.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第S1期89-91,共3页
Academic Journal of Second Military Medical University
关键词
透明质酸
伤口愈合
瘢痕
羟脯氨酸
Ⅲ型前胶原
hyaluronic acid
wound healing
scar
hydroxyproline
procollagen type Ⅲ
