摘要
目的:探讨维拉帕米对梗阻性黄疸时肝组织核因子-κB(nuclear factor kappa B,NF-κB)表达的影响。方法:将雄性SD大鼠48只随机分成胆管结扎组、维拉帕米组和假手术组。分别于术后第7天、14天处死大鼠,观察肝组织NF-κB p65表达、丙二醛(MDA)和超氧化物歧化酶(SOD)含量以及血清肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)的变化。结果:维拉帕米组肝组织NF-κB p65表达、MDA含量及血清TNF-α,IL-6水平均明显低于胆管结扎组(P<0.05或P<0.01)。结论:维拉帕米可下调肝组织NF-κB的表达,减轻梗阻性黄疸时肝损伤。
Objective： To investigate the effect of verapamil on expression of nuclear factor kappa B （NF-κB） in the liver during obstructive jaundice. Methods： Forty eight male Sprague-Dawley rats were randomly divided into three groups： bile duct-ligation group, bile duet-ligation plus verapamil group and sham-operation group. The rats were sacrificed at 7 and 14 days after operation. The following parameters were measured： expressions of hepatic NF-κB p65, contents of maleicdialdehyde （MDA） and superoxide dismutase（SOD） in hepatic tissue, tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） in serum. Results ： Expressions of NF-κB p65, contents of MDA and levels of serum TNF-α, IL-6 in verapamil group were all obviously lower than those in bile duct-ligation group（P 〈 0.05 or P 〈0.01 ）. Conclusion： Verapamil may protect against the liver injury in rats with obstructive jaundice through reducing the expression of NF-κB.
出处
《江苏大学学报（医学版）》
CAS
2009年第2期135-138,I0002,共5页
Journal of Jiangsu University：Medicine Edition
关键词
维拉帕米
梗阻性黄疸
核因子-ΚB
肝损伤
verapamil
obstructive jaundice
nuclear factor kappa B
liver injury
