摘要
目的:探讨电针水沟穴对急性高血压性脑出血模型大鼠脑组织超微结构的影响。方法:参照胶原酶加肝素脑内尾状核联合注射的方法造模。WKY大鼠分为6h、1d、3d、7d对照组。受试SHR大鼠分为模型6h、1d、3d、7d组;假手术6h、1d、3d、7d组;电针6h、1d、3d、7d治疗组。分别在6h、1d、3d、7d用光镜和电镜观察各组大鼠脑组织结构的变化情况。结果:模型组大鼠6h即出现明显的脑组织超微结构改变,模型1d组超微结构病变进一步加重,模型3d组较1d模型组脑组织超微结构病变有所改善,模型7d组超微结构病变与模型6h组相仿。治疗各组与相应模型组比较,大脑皮层神经细胞细胞器肿胀程度减轻,电针7d组,细胞膜结构完整,大部分细胞器结构完整,个别线粒体还存在肿胀,细胞核双层结构,核仁清晰,部分细胞存在异染色质轻微增多现象。结论:电针水沟穴可明显改善高血压性脑出血大鼠脑组织超微结构的损害,这可能是电针水沟穴促进神经功能修复的机制之一。
Objective ：To study the effect of e lectroacupuncture （EA） at Shuigou （RN 26） on ultrastructure in hypertensive intracerebral hemorrhage （ICH） rats. Methods： Experimental hypertensive ICH model of rats were established by injecting collagenase and heparin into right candate nucleus of spontaneously hypertensive rats using stereotaxis technique. WKY rats were divided into 6 hours, 1 day,3 days group and 7 days group after injecting physiological saline into right caudate nucleus, and spontaneously hypertensive rats （SHR） were divided into model groups （6 hours, 1 day,3 days ,7 days） , sham operation groups （6 hours, 1 day,3 days,7 days ） and the EA groups （6 hours, 1 day,3 days,7 days）. Electroacupuncture at Shuigou （RN 26） treated EA groups every day. The changes of brain tissue ultrastructure in every group were observed under light and electron microscope at 6 hours, 1 day,3 days, and 7 days after operation. Results ： In model groups, significant change of brain tissue ultrastructure appeared at 6 hours after operation and the lesion aggravate at 1 days. At 3 days, amelioration of the lesion appeared, and at 7 days the lesion became similar to that of 6 hours group. EA group in all time points showed significant improvement in organelle swelling degree of cerebral cortical neurons compared with the model group, and at 7 days the membrane and most of organelles of nerve cells were perfect, and bilayer structure of nucleus were clear. However, some mitochondria swelled, and there were a little muh heterochromatin in some nerve cells. Conclusion： The therapy of electroacupuncture at Shuigou （RN 26） can improve brain tissue ultrastructure in hypertensive ICH model rat, which may be one of mechanisms of promoting neuronal function restoration.
出处
《江西中医学院学报》
2009年第2期43-46,共4页
Journal of Jiangxi College of Traditional Chinese Medicine
基金
国家自然科学基金资助(30400608
30672714)
上海市重点学科建设资助(T0302)
关键词
电针
水沟穴
脑出血
神经元保护作用
Electroacupuncture
Shuigou （ RN 26）
Intracerebral Hemorrhage
Neuron Protective Effect
