摘要
目的观察不同氧浓度微环境对间充质干细胞增殖的影响,对比分析间充质干细胞在体外常规培养与机体生理微环境中增殖情况的差异。方法从大鼠骨髓中分离获取间充质干细胞;体外培养扩增后,以0.3×104细胞/孔密度接种于96孔培养板;待细胞完全贴壁后,按照实验分组、常规培养组、高压氧组和低压氧组进行处理;采用MTT发连续5d测定各组细胞OD值;统计分析不同氧浓度条件下骨髓间充质干细胞的增殖情况。结果与常规培养组比较,5kPa高压氧组MSCs增长旺盛,第5天的OD值是常规培养组的1.2倍;且随着每次吸氧时间的延长而有增加趋势;2.0kPa高压氧组和低压氧组MSCs表现不同程度的增殖抑制,仅为常规培养组的64%和26%。结论适宜的高浓度氧微环境可以促进大鼠骨髓间充质干细胞的增殖。
Objective To observe the effect of different oxygen concentration microenvironment on the proliferation of rat bone marrow stromal cells（BMSCs） and contrastive analysis the difference of proliferation between the conventional culture and the physiological microenvironment. Methods Culture the MSCs in vitro after obtained from rat bone marrow amplification. The third generation MSCs were seed in the 96 well Cell Culture Plates by 0.3×10^4/well density. The experiment was divided into 3 groups： the conventional culture group, the high pressure oxygen group and the low pressure oxygen group. The OD value was measured with MTT in successive 5 days. Results MSCs proliferation of the 1.5 kPa high pressure oxygen group was 1.2 time higher than the conventional culture group on the fifth day; but the 2.0 kPa high pressure oxygen group and the Low Pressure Oxygen group was 64% and 26% lower than the conventional group. Conclusion The eligible high oxygen concentration microenvironment culture can promote the proliferation of rat bone marrow stromal stem cells.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第8期891-893,共3页
Chongqing medicine
基金
重庆市自然科学基金项目(CSTC
2006BB5062)
关键词
间充质干细胞
氧浓度
微环境
增殖
MTT
BMSCs
oxygen concentration
microenvironment
proliferation
MTT
