摘要
目的评价结膜下注射贝伐单抗(bevacizumab)对大鼠碱烧伤角膜新生血管化的影响。方法将22只成年雄性Wistar大鼠,碱烧伤诱导单眼角膜新生血管模型后随机分为两组:实验组和对照组。实验组(10眼)给予贝伐单抗原液(25mg/ml)0.02ml结膜下注射,对照组(10眼)给予结膜下注射等量生理盐水,两组给药时间均为碱烧伤第1天、第3天及第5天,每日1次,共3次。分别采用宏观测量新生血管长度、生长速度及新生血管化面积,显微镜下微血管计数方法研究角膜新生血管形成及抑制情况。结果实验组新生血管生长速度较对照组慢[(1.05±0.62)mm/dvs(2.87±0.34)mm/d,P<0.05];新生血管化面积实验组较对照组小[(30.5±18.8)%vs(57.5±11)%,P<0.05];微血管计数实验组较对照组少,差异均具有统计学意义(1.86±1.54vs8.51±2.33,P<0.05)。结论贝伐单抗可抑制大鼠碱烧伤角膜新生血管的增殖。
Objective To evaluate the effects of the subconjunctival injection of bevacizumab on angiogenesis in alkali burn cornea of rats. Methods The corneal neovascularization（CNV） model was induced by alkali burns in corneas of 22 Wistar male rats. And then they were divided in two groups ： control group and experimental group. The corneas in control group received subconjunctivally 0.02 ml of 0.9% saline solution, while the corneas in experimental group received subconjunctivally 0.02 ml of bevacizumab（ 25 mg/ml）at day 1,3 and 5 after lesion. The angiogenesis and inhibition of corneal neovascularization （CNV）were detected by calculating microvessl numbers under microscopy, and determining the length,the growth rate and the area of CNV under sht lamp. Results The growth rate of CNV was slower in experimental group than in control group [ （ 1.05± 0.62） mm/d vs （2.87± 0.34） mm/d, P 〈 0.05 ]. The area of neovascularization of the corneal surface in experimental group was smaller than in control group[ （30.5 ± 18.8 ） % vs （57.5 ± 11 ） %, P 〈 0.05 ]. The microvessl number was lower in experimental group （ 1.86 ± 1.54 ） than in control group （ 8.51 ± 2.33, P 〈 0.05 ）. Conclusion Bevacizumab may inhibit corneal angiogenesis.
出处
《山西医科大学学报》
CAS
2009年第4期326-328,F0003,共4页
Journal of Shanxi Medical University
关键词
贝伐单抗
角膜
新生血管
bevacizumab
cornea
neovascularization
